Nuestro último artículo, aceptado en Journal of Applied Ichthyology

Japanese eel (Anguilla japonica Temminck & Schlegel, 1846) propagation by using cryopreserved sperm samples

Tamás Müller, Hajime Matsubara, Yuki Kubara, Ákos Horváth, Juan F. Asturiano, Béla Urbányi

Abstract

Our aim of this study was to test the artificial seminal plasma (ASP) as extender and methanol as cryoprotectant for cryopreservation and to collect information on the fertilizing capacity of cryopreserved sperm samples. Eggs from Japanese eel females were controlled fertilized with native sperm diluted with ASP and cryopreserved sperm (ASP as extender (in 1:100 ratio) and 10% methanol in v/v final concentration). There were no statistical differences (p<0.05) among the measured parameters (hatching, survival rates after 10 days post-hatch, malformation) between two groups so this cryopreservation method can be effectively used for artificial propagation of Japanese eel as well.