martes, 28 de junio de 2016

Asistimos al VI Congreso Ibérico de Ictiología


Entre el 21 y el 24 de junio se ha desarrollado en Murcia el VI Congreso Ibérico de Ictiología: vocación por los peces de mar, los peces de río y la acuicultura, organizado por la Sociedad Ibérica de Ictiología (SIBIC) y por el grupo de Mar Torralva de la Universidad de Murcia.
En el congreso ha constado de 111 sesiones orales, incluyendo 6 sesiones plenarias (entre las que destacaron la de Ibon Cancio y la de Fernando de la Gándara), y de casi 100 posters. Ha contado con 204 participantes de 15 países diferentes.

Hemos acudido cuatro miembros del Grupo de Acuicultura y Biodiversidad de la UPV (junto a Carina Caldeira en la foto). Miguel Jover abrió la primera sesión de Acuicultura, Endocrinología y Toxicología que se realiza en este Congreso, y que se prolongó durante los tres días del mismo, con una charla titulada: “Situation, prospects and challenges of aquaculture in the Iberian Peninsula”.
Víctor Gallego participó en esta sesión con una comunicación oral titulada: “Sperm quality in fish: factors to consider for application in aquaculture”, mientras que David Sánchez Peñaranda participó con su oral titulada: “Temperature modulates eel maturation”.

Por mi parte, tuve ocasión de participar con una comunicación oral titulada: "Reproduction of European eel in captivity: Where we are?” y en una mesa redonda posterior en la  Sesión Especial: Retos y oportunidades en la investigación de la anguila europea, en la que se hizo un repaso a diversos aspectos relacionados con la realidad de la situación de esta especie en la Península Ibérica y en el resto de Europa.


El Congreso ha sido interesante y variado, y difícilmente superable en cuanto a organización. Muchas gracias a nuestros colegas de Murcia!. Y enhorabuena a la SIBIC, que sigue creciendo.

lunes, 20 de junio de 2016

Nuestro último artículo, aceptado en Comparative Biochemistry and Physiology - Part A: Molecular & Integrative Physiology


Identification of the major proteins present in the seminal plasma of European eel, and how hormonal treatment affects their evolution. Correlation with sperm quality
M. Carmen Vílchez, Davinia Pla, Víctor Gallego, Libia Sanz, Luz Pérez, Juan F. Asturiano, Juan J. Calvete, David S. Peñaranda

Abstract
By first time, 2DE protein profile of European eel seminal plasma has been determined. 14 different proteins corresponding to 9 major families were identified in seminal plasma, through hormonal treatment. Some of them play a part in sperm maturation, including carbonic anhydrase which is responsible for modulating the pH of seminal plasma, and warm temperature acclimation protein, which may play an important role in the final maturation of this species, due to the warm temperature of their spawning ground (in the Sargasso Sea).
Sperm samples were classified into three motility categories depending on the percentage of motile cells, I: 0-25%, II: 25-50% and III: >50%. Different protein profiles were observed depending on the sperm motility categories, specifically, with the apolipoproteins and complement C3. Higher numbers of proteins from the apolipoprotein family were registered at lower motilities; whereas the complement C3-like family was higher in the samples with the highest percentage of motile cells. These results suggest that the proteins linked to the transportation of lipids (apolipoprotein) and to the immune system (complement C3) may carry out their functions at different stages of spermatogenesis. Using SDS-PAGE analysis, 13 bands were identified, most of which migrated between 20 to 60 kDa. In the last weeks of treatment significant increases were observed in the percentage of motile spermatozoa, curvilinear velocity and beat cross frequency. This improvement in sperm quality coincided with a higher amount of proteins located at 19 KDa, therefore, this protein could be involved in sperm motility of the European eel.

Doi:10.1016/j.cbpa.2016.06.025

domingo, 12 de junio de 2016

Nuestro último artículo, aceptado en General and Comparative Endocrinology


Progress, challenges and perspectives on fish gamete cryopreservation: A mini-review

Juan F. Asturiano, Elsa Cabrita, Ákos Horváth

Abstract
Protocols for the cryopreservation of fish gametes have been developed for many different fish species, in special, freshwater salmonids and cyprinids. Methods for sperm freezing have progressed during the last decades due to the increasing number of potential applications: aquaculture (genetic improvement programs, broodstock management, helping with species having reproductive problems), biotechnology studies using model fish species (preservation of transgenic or mutant lines), cryobanking of genetic resources from endangered species, etc.
This mini-review tries to give an overview of the present situation of this area of research, identifying the main challenges and perspectives, redirecting the reader to more in-depth reviews and papers.

martes, 7 de junio de 2016

Manifiesto “Ríos sin barreras”


Por iniciativa de la Sociedad Ibérica de Ictiología (SIBIC)  en colaboración con la Asociación para el Estudio y Mejora de los Salmónidos (AEMS – Ríos con Vida), la Asociación Ibérica de Limnologia (AIL), el Centro Ibérico de Restauración Fluvial (CIREF), la Sociedad Española de Ornitología (SEO/Birdlife), la World Fish Migration Foundation (WFMF) y el World Wildlife Found (WWF), se ha enviado a los medios de comunicación el manifiesto Ríos sin barreras que podeis encontrar en la web de la SIBIC: http://www.sibic.org/manifiesto-rios-sin-barreras/

Os pedimos a todos que nos ayudéis a darle la máxima difusión! 

viernes, 3 de junio de 2016

Announcement 7th AQUAGAMETE Training School (Fish proteomics)

The AQUAGAMETE COST Action (aquagamete.webs.upv.es/web) is pleased to announce the 7th AQUAGAMETE Training School.

The 7th AQUAGAMETE Training School is aimed towards young researchers (PhD students, young post-docs and MSc students) as well as representatives from industry. The course will be focused on Fish proteomics and will include theoretical and practical classes. The course will provide an introduction to the field of proteomics and emphasizes hands-on experience with two dimensional electrophoresis and mass spectrometry protein identification.

Specific topics include: Introduction to proteomics; Proteomics in reproductive science; Proteomics in fish research; Techniques of sample preparation for proteomic analysis; Two dimensional electrophoresis; Detection of spots, digestion and protein identification; Bioinformatics in proteomic research

The 7th AQUAGAMETE Training School will highlight the most recent developments in each topic, promoting a strong interaction between experienced trainers working in different proteomic areas and trainees.

The course will take place between 26 - 30th September 2016 at the Institute of Animal Reproduction and Food Research in Olsztyn, Poland.

The number of participants will be restricted to 9 persons. Financial support from the AQUAGAMETE COST Action (for travelling, meals and accommodation) will be available for trainees. Registration will be available from 1st August and both for students (no registration fee) and for industry representatives (fee of 300 euros) and includes access to all lectures and laboratorial practices, support material and transport between facilities and main hotel.

The application deadline is 10th July and complete applications must be sent via e-mail to aquagamete@gmail.com. Eligible candidates will be announced by the end of July.

For more detailed information please contact Andrzej Ciereszko (a.ciereszko@pan.olsztyn.pl).

Applications should include:
  • Application form
  • short Curriculum Vitae in the area (only for students)
  • motivation letter for participating in the training school, which should indicate the academic/professional status of the applicant
  • letter of recommendation from the applicant’s supervisor or employer
  • Financial support justification where candidates should estimate their costs in travelling, accommodation and meals during their attendance to the Training School.

We would appreciate if you could forward this announcement via e-mail to colleagues or students who may be interested in participating. More information available soon at:  http://aquagamete.webs.upv.es/

jueves, 2 de junio de 2016

Celebramos el Día Mundial de la Migración de los Peces

El pasado sábado 21 de mayo el Grupo de Acuicultura y Biodiversidad (GAB) de la Universitat Politècnica de València celebró el Día Mundial de la Migración de los Peces (World Fish Migration Day) en el Centro de Interpretación Racó de l’Olla, en el Parque Natural de La Albufera de Valencia. Este evento se realizó en el marco de la Red de Formación Europea (European Training Network) IMPRESS (Marie Sklodowska-Curie Actions, H2020), en la que participamos.

El objetivo del World Fish Migration Day  es concienciar a la población de la importancia de los ríos en la migración de los peces, que deberían poder nadar por ellos sin encontrar obstáculos como presas o azudes construidos por el hombre que impiden sus movimientos migratorios. La primera edición tuvo lugar en mayo de 2014 y en ella se organizaron un total de 270 eventos en todo el mundo. Sin embargo, en esta segunda edición la cifra ha alcanzado los 450 eventos en 61 países, registrándose unos 15 millones de seguidores en las redes sociales.

Cerca de 240 personas visitaron el Centro de Interpretación el día 21 a lo largo de la mañana, y disfrutaron de las actividades organizadas. En primer lugar, tuvieron la oportunidad de escuchar las charlas informativas a cargo del Dr. Asturiano, quien presentó al público el World Fish Migration Day y sus objetivos, el ciclo reproductivo de la anguila europea y sus problemas en acuicultura, cómo el GAB trata de ayudar con sus experimentos en la UPV y cómo el proyecto IMPRESS trata asimismo de ayudar a otras especies migradoras como el salmón atlántico y los esturiones, muchos de ellos en peligro de extinción.


Los visitantes pudieron ver ejemplares adultos (machos y hembras) de anguila, así como angulas.



Después de cada charla, la cuentacuentos Lorena Comín, de Disparatario, narró la historia amenizada con juegos de “Ann Guillidae”, escrita por Eva Lucía Bayarri e ilustrada por Susana Vilanova. La historia cuenta cómo una pequeña angula (Ann) llega a la Albufera y conoce a un samaruc (Valencia hispanica), Sam. Ann habla a Sam sobre su largo viaje desde el Mar de los Sargazos y sobre su intención de quedarse allí, en agua dulce, hasta que vuelva a partir hacia el lugar donde nació. Así, de una forma didáctica, los jóvenes asistentes aprendieron cómo es el ciclo vital de la anguila europea.




Los niños pudieron colorear divertidos dibujos de peces migradores, la mayoría de ellos proporcionados por la plataforma de World Fish Migration Day, y opcionalmente, participar en su concurso de dibujo.

Finalmente, pudieron visitar uno de los sitios por donde las anguilas entran y salen de la Albufera al mar, la Gola de El Pujol. El Taller de Empleo Conservación del Bosque Devesa Albufera II del Ayuntamiento de Valencia colaboró realizando visitas guiadas a lo largo de la “Gola de El Pujol”, durante las cuales explicaron a los visitantes detalles interesantes sobre la Albufera, la pesca tradicional de angulas, las aves que se pueden avistar, las compuertas que regulan el nivel del agua de la Albufera en función de las necesidades del cultivo de arroz, etc.
En general, los visitantes disfrutaron de este evento, y muchos de ellos reconocieron que no sabían que la anguila que tradicionalmente se había estado utilizando en la gastronomía local desde hace tantísimo tiempo era una criatura tan excepcional.

Nuestro agradecimiento al personal del Centro de Visitantes Racó de l´Olla, a los miembros del Taller de Empleo Conservación del Bosque Devesa Albufera II del Ayuntamiento de Valencia, a la Cofradía de Pescadores de El Palmar, a la Generalitat Valenciana y a la ETN IMPRESS.

lunes, 23 de mayo de 2016

Nuestro último artículo, aceptado en General and Comparative Endocrinology

Development of sperm vitrification protocols for freshwater fish (Eurasian perch, Perca fluviatilis) and marine fish (European eel, Anguilla anguilla)

Eszter Kása, Gergely Bernáth, Tímea Kollár, Daniel Żarski, Jelena Lujić, Zoran Marinović, Zoltán Bokor, Árpád Hegyi, Béla Urbányi, M. Carmen Vílchez, Marina Morini, David S. Peñaranda, Luz Pérez, Juan F. Asturiano, Ákos Horváth

Abstract
Vitrification was successfully applied to the sperm of two fish species, the freshwater Eurasian perch (Perca fluviatilis) and marine European eel (Anguilla anguilla). Sperm was collected, diluted in species-specific non-activating media and cryoprotectants and vitrified by plunging directly into liquid nitrogen without pre-cooling in its vapor. Progressive motility of fresh and vitrified-thawed sperm was evaluated with computer-assisted sperm analysis (CASA). Additional sperm quality parameters such as sperm head morphometry parameters (in case of European eel) and fertilizing capacity (in case of Eurasian perch) were carried out to test the effectiveness of vitrification. The vitrification method for Eurasian perch sperm resulting the highest post-thaw motility (14 ± 1.6%) was as follows: 1:5 dilution ratio, Tanaka extender, 30% cryoprotectant (15% methanol + 15% propylene-glycol), cooling device: Cryotop, 2 μl droplets, and for European eel sperm: dilution ratio 1:1, with 40% cryoprotectant (20% MeOH and 20% PG), and 10% FBS, cooling device: Cryotop, with 2 μl of sperm suspension. Viable embryos were produced by fertilization with vitrified Eurasian perch sperm (neurulation: 2.54±1.67%). According to the ASMA analysis, no significant decrease in head area and perimeter of vitrified European eel spermatozoa were found when compared to fresh spermatozoa.

Doi:10.1016/j.ygcen.2016.05.010


martes, 3 de mayo de 2016

Día Mundial de la Migración de los Peces (World Fish Migration Day)


El próximo 21 de mayo se celebrará en el Centro de Interpretación Racó de l’Olla, en el Palmar, una jornada de divulgación sobre la anguila europea. Esta actividad se realiza en el marco del Día Mundial de los Peces Migradores (World Fish Migration Day, www.worldfishmigrationday.com) y de la Acción Europea Marie Sklodowska-Curie IMPRESS (http://www.impress-itn.eu/). Se trata de un acontecimiento internacional en el que se  desarrollarán cerca de 300 eventos en todo el mundo, para concienciar a la población sobre la situación de los peces migradores y de nuestros ríos y lagos, su papel ecológico y gran importancia. En mayo de 2014 tuvo lugar la primera edición, y en esta segunda se espera superar las cifras de participación.
El Grupo de Acuicultura y Biodiversidad de la Universitat Politècnica de València, especializado en el estudio de la reproducción de esta especie (http://acuiculturaenvalencia.blogspot.com.es/), ha organizado actividades, dirigidas tanto a grandes como a pequeños, que consistirán en:
- Charlas para dar a conocer las peculiaridades del ciclo vital de la anguila (a las 10:00, 11:00, 12:00 y 13:00);
- Exposición de ejemplares vivos y artes de pesca locales;
- Concurso de dibujo y coloreado con la temática de los peces migradores (a cargo de la plataforma World Fish Migration Day);
- Narración del cuento “Ann Guillidae”, escrito por Eva Lucía Bayarri e ilustrado por Susana Vilanova, a cargo de la Cuentacuentos Lorena Comín (pases a las 10:20, 11:20, 12:20 y 13:20);

- Visitas guiadas por el Itinerario de la Gola de El Pujol, uno de los sitios por donde la anguila entra y sale de la Albufera, en colaboración con el servicio Devesa-Albufera del Ayuntamiento de Valencia (a las 11:00, 12:00 y 13:00).



jueves, 21 de abril de 2016

Nuestro último artículo, aceptado en Aquaculture

Hormonal manipulations for the enhancement of sperm production in cultured fish and evaluation of sperm quality

Constantinos C. Mylonas, Neil J. Duncan, Juan F. Asturiano

Abstract
This article reviews the use of hormonal treatments to enhance sperm production in aquaculture fish and the methods available for evaluating sperm quality. The different types of testis development are examined and a brief review is presented of the endocrine regulation of spermatogenesis in fishes, including the increasing evidence of the existence of spermatozoa subpopulations. Hormonal manipulations are employed to induce spermatogenesis in species such as the freshwater eels, to synchronize maximal sperm volume to ovulation for in vitro fertilization and to enhance sperm production in species with poor spermiation. The hormones that are employed include gonadotropins (GtH) of piscine or mammalian origin, and gonadotropin-releasing hormone agonists (GnRHa) administered by injections or controlled-release delivery systems, with or without dopaminergic inhibitors.
Pheromones in the culture water and hormones added to the sperm in vitro have also been employed to enhance spermiation and sperm quality, respectively, in some fishes. Hormonal therapies usually do not affect sperm quality parameters, except in cases where fish fail to spermiate naturally or produce very small volumes of high-density sperm. Different parameters have been used to evaluate fish sperm quality, including sperm volume and density, spermatozoa motility and morphometry, and seminal plasma composition. The development of Computer-Assisted Sperm Analysis (CASA) systems made possible the estimation of a higher number of sperm motion parameters using an objective, sensitive and accurate technique. The development of Assisted Sperm Morphology Analysis (ASMA) software has introduced a new approach for sperm evaluation studies, demonstrating changes in the spermatozoa related to reproductive season, hormonal treatments or the cryopreservation processes, and how these may be related to changes in sperm motility and fertilization capacity. The article concludes with a few practical protocols for the enhancement of sperm production in aquaculture species.

Available here: http://authors.elsevier.com/sd/article/S004484861630206X

miércoles, 13 de abril de 2016

Nuestro último artículo, aceptado en Reproduction in Domestic Animals


First production of larvae using cryopreserved sperm: Effects of preservation temperature and cryopreservation on European eel sperm fertilization capacity

Juan F. Asturiano, Sune R. Sørensen, Luz Pérez, Peter Lauesen, Jonna Tomkiewicz

Abstract

Sperm cryopreservation is a useful tool in captive fish reproduction management, i.e. in order to synchronize gamete production, especially in the case of species as the European eel, where the time of female spawning readiness is unpredictable. Several protocols to cryopreserve sperm of this species have been described, but until recently fertilization trials were not feasible. The present study evaluated the effect of cold storage of diluted sperm prior to fertilizations and tested if a previously defined protocol for European eel sperm cryopreservation can be successfully applied in fertilization trials to produce viable offspring. In our experiment, the sperm motility was evaluated after the extraction and the best samples were selected and pooled. Until stripping of eggs and fertilization, diluted sperm samples were maintained at either 4 or 20 ºC, or cryopreserved, following existing protocols. Fertilization of two egg batches was attempted. Diluted sperm caused a similar percentage of fertilized eggs and a similar number of embryos and larvae, independently of storage temperature (4 or 20 ºC). The cryopreserved sperm resulted in a lower percentage of fertilized eggs, but embryos developed and a few larvae (“cryolarvae”) were obtained 55 h after fertilization in one of the two egg batches. This result evidences that the tested cryopreservation protocol is applicable for eel reproduction management, although improvements will be required to enhance fertilization success.

Oferta de contrato predoctoral en Berlín dentro del Proyecto IMPRESS

The Leibniz-Institute of Freshwater Ecology and Inland Fisheries (www.igb-berlin.de) is the largest research institute for freshwater research in Germany. It is member of the Leibniz Association and the Forschungsverbund Berlin e.V. IGB has close links to all three universities in the German capital and currently hosts about 50 doctoral students from approximately 15 different nations. Since 1996, the IGB has been committed to research on sturgeon remediation and restoration in the tributaries of the Baltics and the North Sea, including several national and international projects and programmes. Within the EU Marie Sklodowska-Curie Initial Training Network IMPRESS (Improved production strategies for endangered freshwater species, http://www.impress-itn.eu), the Research Group Applied Fish Physiology and Aquaculture led by Dr. Sven Würtz is looking for a hard-working, enthusiastic
Early-stage researcher
Trait-related optimization of hatchery technology to improve fitness and stocking efficiency in sturgeon
This is position 7 within IMPRESS, available from now on until 31.08.2018

IMPRESS aims at developing new technologies for improved production, management and conservation of threatened iconic diadromous fish species. IMPRESS is set up as intersectoral network of 15 ESR hosted by 9 different hosts covering molecular biology, physiology, and aquaculture technology, as well as human dimensions of biodiversity conservation. The project will focus on assessing and improving fitness of hatchery-reared early life stages in the wild. Challenge tests will be carried out to characterize ecophysiological adaptations to fluctuating environmental challenges and improve aquaculture technologies in conservation aquaculture. During the project the ESR will spend up to 6 months at the Norwegian University of Life Sciences (host Ian Meyer) assessing impaired cognitive ability and behaviour in hatchery of sturgeon. Finally the student will carry out transcriptome analysis of selected samples at the ZF Screens (Ron Dirks) in Leiden, Netherlands. This mobility will enhance multidisciplinary training of the student, and give him/her important experience of both academic and non-academic sectors (training school programme).
More details on the topic, the ITN network, the location, the team of supervisors and their groups, the expected mobility, salary and general eligibility criteria can be found in a long version of this job advertisement posted at www.igb-berlin.de/job-offers.html.
Applicants should have a 2.1 degree or equivalent in a relevant life science (biology, fisheries sciences, aquaculture and environmental sciences). Excellent communication skills in English are required. Experience with molecular techniques (e.g. qPCR, transcriptome analysis) and analytical chemistry (mass spectrometry, chromatography) is highly preferred. Candidates with a background in aquaculture (particularly larval rearing) will be deemed highly competitive.
According to EU regulations candidate students must not have resided for more than 12 months during the previous 3 years in the prospective host country, Germany.
Salary is paid according to the regulations of the programme. In keeping with the IGB's policy regarding gender equity, female applicants are particularly encouraged. Among candidates of equal aptitude and qualifications, a person with disabilities will be given preference.

Review of applications meeting the required standards will start immediately and continue until a candidate is appointed. Please send your application and further inquiries to Dr. Sven Würtz (wuertz@igb-berlin.de).

La SIBIC estrena nueva web


La Sociedad Ibérica de Ictiología cumplió 5 años el pasado diciembre de 2015 y ha renovado su web (www.sibic.org). La nueva web es mucho más moderna y dinámica. Se ha construido para que sea totalmente accesible desde ordenadores, tablets y smartphones. También se han integrado la web de la revista Fishes in Mediterranean Environments (www.fishmedjournal.org) y los congresos celebrados hasta el momento por la SIBIC. Además se ha añadido la versión en inglés para facilitar y fomentar la visibilidad y las relaciones internacionales.
Os invitamos a navegar por la nueva sibic.org


martes, 12 de abril de 2016

Nuestro último artículo, aceptado en Comparative Biochemistry and Physiology - Part A: Molecular & Integrative Physiology


Sodium affects the sperm motility in the European eel

M. Carmen Vílchez, Marina Morini, David S. Peñaranda, Víctor Gallego, Juan F. Asturiano, Luz Pérez

Abstract

The role of seminal plasma sodium and activation media sodium on sperm motility was examined by selectively removing the element from these two media, in European eel sperm. Sperm size (sperm head area) was also measured using an ASMA (Automated Sperm Morphometry Analyses) system, in the different conditions. Intracellular sodium [Na+]i was quantitatively analyzed by first time in the spermatozoa from a marine fish species. Measurement of [Na+]i was done before and after motility activation, by Flow Cytometry, using CoroNa Green AM as a dye. Sperm motility activation induced an increase in [Na+]i, from 96.72 mM in quiescent stage to 152.21 mM post-activation in seawater. A significant decrease in sperm head area was observed post-activation in seawater. There was a notable reduction in sperm motility when sodium was removed from the seminal plasma, but not when it was removed from the activation media. Sodium removal was also linked to a significant reduction in sperm head area in comparison to the controls. Our results indicate that the presence of the ion Na+ in the seminal plasma (or in the extender medium) is necessary for the preservation of sperm motility in European eel, probably because it plays a role in maintaining an appropriate sperm cell volume in the quiescent stage of the spermatozoa.

martes, 5 de abril de 2016

Nueva Training School de AQUAGAMETE

The AQUAGAMETE COST ACTION is pleased to announce the 6th AQUAGAMETE training school which will be focused on the Molecular basis of gamete quality and reproduction, with a strong orientation towards GENOMIC TOOLS

The course will be organized by the Fish Physiology and Genomics Department of INRA, Rennes (France) (coordination by Julien Bobe and Catherine Labbé), and will be held in Rennes from June 6th to 10th 2016.

The course will include conferences on:
- Sperm genetic damage and repair in Fish (Paz Herraez, Spain)
- Overview of sequencing technologies and strategies available to analyze fish reproduction (Yann Guiguen, France)
- Epigenetics and inheritance (Francesc Piferrer, Spain)
- Transgenesis approaches in fish reproduction (speaker to be determined)
- CrispR/Cas9 technology for research on fish reproduction (Amaury Herpin, France)
- microRNA and female fecundity (Amine Bouchareb, France)

The practical sessions will be coordinated by the Fish Physiology and Genomics staff.  Include experiment on: 
- RNA extraction (2h30)
- Microarray analysis of fish samples in reproduction research (10h)
- Sequence searching including tools presentation, primer design, phylogeny/syntheny search (5 h)

The number of participant is restricted to 16 persons. Priority will be given to early stage researchers (MSc students, PhDstudents, young post-docs) and to participants with no experience in genomics and with a professional project related to gamete quality or fish reproduction, including representative from industry.

Financial support from the AQUAGAMETE COST action for travelling, accommodation and meals will be available for students. The access to lectures, laboratory facilities and support material is supported for all participants by the AQUAGAMETE COST action and by the host Fish Physiology and Genomics department.

The application deadline is May 2nd, 2016. Complete applications must be sent via e-mail to aquagamete@gmail.com. Eligible candidates will be announced by May 6th. For more detailed information please contact Pepa Bayarri (aquagamete@gmail.com).

Applications should include the following documents:
• Application form (attached);
• Short Curriculum Vitae in the area;
• Motivation letter for participating in the training school, which should indicate the academic/professional status of the applicant;
• Letter of recommendation from the applicant’s supervisor or employer;
• Financial Support justification (only for students).

More information available soon at: http://aquagamete.webs.upv.es


martes, 29 de marzo de 2016

Retos y oportunidades en la investigación de la anguila europea

Este será el título de una de las Sesiones Especiales del VI Congreso Ibérico de Ictiología (Murcia, España, 21 al 24 de junio de 2016; www.um.es/sibic6).
La población de la anguila europea ha sufrido un intenso y continuo declive durante las últimas décadas; el reclutamiento ha bajado en el 2015 comparado a 2014, de un 3.7 % del nivel histórico a un 1.2 en el área del Mar del norte y de un 12.2 a un 8.4% en el resto de Europa (ICES/EIFAC/GCFM 2015). Durante los últimos años se han establecido diversas medidas de gestión para garantizar la conservación de esta emblemática especie. Sin embargo, existe una gran incertidumbre respecto a la capacidad de recuperación de la especie debido a que aún hoy en día no se conocen en su totalidad los procesos que condicionan la situación de la población.
El objetivo de esta sesión es, teniendo en cuenta la situación de la población, identificar cuáles son los retos a los que nos enfrentamos y las oportunidades que nos ofrece la investigación de la anguila actualmente, incluyendo diversos aspectos como control de poblaciones, patología, parasitología y control de la reproducción en cautividad.
Para ello, especialistas ibéricos en anguila realizarán una síntesis sobre la situación de la especie, sus principales amenazas actuales y potenciales, el efecto de las medidas de gestión aplicadas hasta el momento y los últimos avances en su investigación.
Finalmente, se llevará a cabo una mesa redonda en la que los especialistas y asistentes, utilizando la información de las presentaciones, podrán discutir sobre los retos e identificar las oportunidades que presenta la investigación sobre esta fascinante especie.

lunes, 21 de marzo de 2016

Nuestro último artículo, aceptado en Comparative Biochemistry and Physiology - Part A

Temperature modulates testis steroidogenesis in European eel

David S. Peñaranda, Marina Morini, Helge Tveiten, M. Carmen Vílchez, Víctor Gallego, Ron P. Dirks, Guido E.E.J.M. van den Thillart, Luz Pérez, Juan F. Asturiano

Abstract
This study evaluates the effects of temperature on hCG-induced spermatogenesis in European eel (Anguilla anguilla), subjected to three thermal regimes: T10: 10 °C (first 4 weeks), 15 °C (next 3 weeks) and 20 °C (last 6 weeks); T15: 15 °C (first 4 weeks) and 20 °C (last 9 weeks); and T20: constant 20 °C for the duration of the experiment. At 10 ºC, maturation stopped in the A spermatogonial stage (SPG1), and no further maturation was observed until the temperature was ≥15 ºC. With the aim of explaining these results, the influence of temperature on steroidogenic enzyme gene expression and steroid synthesis was tested. The initial synthesis of androgens (T and 11-KT) increased at SPG1, and was not influenced by temperature. Likewise, the gene expression of the steroidogenic enzymes linked to androgen synthesis (aacyp11a1, aacyp17-I and aa11βHSD) also increased at SPG1. In contrast, no correlation was seen between the increase in E2 and the aacyp19a1 gene expression peak in the testes, with E2 increasing as a consequence of the seawater acclimation carried out before hormonal treatment, and peaking the aacyp19a1 gene expression at B spermatogonial stage (SPG2).  Aacyp21 gene expression was also higher at SPG2, and this stage was only reached when the rearing temperature was ≥15 ºC.

In conclusion, androgen synthesis is not dependent on temperature, but further maturation requires higher temperatures in order to induce a change in the steroidogenic pathway towards oestrogen and progestin synthesis. This study demonstrates that temperature plays a crucial role in European eel maturation, even perhaps controlling gonad development during the reproductive migration.


Luka Glamuzina termina su estancia con nosotros

Hace unas semanas, Luka Glamuzina (bajo a la derecha en la foto), estudiante de la University of Split (Croacia) acabó una estancia de 5 meses (30 septiembre 2015-28 febrero 2016) financiada por el programa Erasmus+ en nuestro grupo de investigación.

Ha participado en diferentes trabajos durante este periodo, en especial en el cuidado de las distintas especies de peces con las que trabajamos en nuestro laboratorio. Ha sido un placer trabajar con alguien siempre alegre y dispuesto a colaborar. Muchas gracias Luka!. 

Curso sobre el uso de zebrafish

Using zebrafish as a model in biomedicine and study of human diseases – 2nd Edition

The course will provide basic knowledge on the use of the zebrafish as a biological model, with main emphasis on its use for the study of human diseases and in research in biology and biomedical sciences. An introduction to genetic manipulation and production of transgenic lines and “knock-out” will be provided. It is intended to provide the students with skills for using the zebrafish in genetic manipulation techniques, use of transgenic and mutants and studies using microinjection and tissue regeneration.

In CCMAR, Faro (Portugal); May 12, 2016.

Registrations: ATT.CCMAR.UALG.PT/ZEBRAFISH_MAY2016/INDEX.HTML 

lunes, 14 de marzo de 2016

30th ESCPB Congress


New European Society for Comparative Physiology and Biochemistry. 4-7 septiembre 2016. Barcelona (España). http://www.escpb.eu/

The theme of the congress is ‘Unraveling complexity: from molecules to ecosystems’.

The innovative use of chemicals and nanomaterials in new technologies, industry and agriculture, in a context of global warming/acidification, challenges many aspects of the ecosystems functioning. An in-depth knowledge of the environment, the biological responses from molecules to ecosystems, and the physiological adaptations of the organisms is needed and further challenges the scientific community to find out innovative solutions. The conference will be an ideal platform where to present, discuss and implement this knowledge and to point out future needs. An attractive scientific program encompassing 8 different scientific sessions will be developed over 4 days.

miércoles, 17 de febrero de 2016

5 años de blog

Lo que empezó en febrero de 2011 como un pequeño experimento a ver cómo se me daba esto de la comunicación on-line, se ha ido consolidando, y van ya 5 años!. Parece difícil teniendo en cuenta todas mis limitaciones en el uso avanzado de herramientas informáticas más allá del nivel de usuario patoso (sirva esto para animar a cualquiera que se haya planteado esto de escribir un blog).

Pero lo más importante es que el registro dice que el número acumulado de visitas pasa de las 49.000. También anima mucho el hecho de que varios colegas me han dicho que echan un vistazo por aquí de vez en cuando. No se trataba de dar una gran información científica, sino más bien de tener un escaparate de las múltiples actividades de un grupo de investigación como el nuestro, o de algunas novedades y próximos eventos. Si a algunos os va siendo útil, me alegro mucho. Gracias por estar ahí!.


lunes, 15 de febrero de 2016

Se cierra el programa de nuestras próximas Training Schools (IMPRESS-AQUAGAMETE)



The 2nd IMPRESS Workshop and the 5th AQUAGAMETE Training School will be held in Valencia (Spain), from 1st to 11th of March, 2016, as joint events of two European networks, the Marie Sklodowska Curie Action Innovative Training Network IMPRESS (http://www.impress-itn.eu/), and the AQUAGAMETE COST Action (http://aquagamete.webs.upv.es/).

More info: http://trainingschoolvalencia.weebly.com/

lunes, 1 de febrero de 2016

International Summer School 2016

La Faculty of Fisheries and Protection of Waters de la University of South Bohemia in České Budějovice (FFPW USB) ha organizado su International Summer School 2016 que se llevará a cabo en la República Checa del 27 de junio al 22 de julio de 2016.

La Summer School  incluye excursiones y clases teóricas, pero sobre todo consiste en trabajar en pequeño Proyecto de investigación bajo la supervisión de investigadores experimentados en los laboratorios de la Facultad, muy bien equipados. Los profesores serán destacados investigadores checos o de otros países.

La FFPW USB proporciona alojamiento y pensión completa gratis a los participantes. El trabajo en los laboratorios será suplementado con varias excursions a piscifactorías y visitas a otros lugares de interés.

Más información (registro, contacto, etc) en su website.

Los estudiantes interesados en la International Summer School 2016 deben enviar un CV, una carta de motivación y un impreso de solicitud a la responsable: Bc. Dana Brožová – dbrozova@frov.jcu.cz  hasta el 31 de marzo.

Desde la UPV hemos enviado a dos alumnas en ediciones anteriores y en ambos casos les ha parecido muy interesante y han disfrutado de la experiencia que ofertan cada año estos colegas de la República Checa. Animáos.

jueves, 21 de enero de 2016

Elvira Mayo defiende su Tesis Doctoral

La semana pasada participé como vocal del tribunal de la Tesis Doctoral de Elvira Mayo Hernández (muy sonriente en la foto con sus codirectoras a los lados, y el resto de miembros del tribunal, las Dras. Elena Barcala, del IEO de San Pedro del Pinatar, y Juana Ortiz, de la UMU, que actuó como Presidenta del tribunal) en la Facultad de Veterinaria de la Universidad de Murcia. Su trabajo se titula: “Comunidad de parásitos de la anguila europea (Anguilla anguilla) del Mar Menor y evaluación de sus efectos en la aptitud ecológica de la población”, ha sido presentado como un compendio de artículos previamente publicados, y ha sido codirigida por las Dras. Pilar Muñoz y Rocío Ruiz de Ybáñez.

En el trabajo se estudian los distintos parásitos de la anguila en el Mar Menor y se concluye que tienen muchos menos nematodos parásitos Anguillicoloides crassus que otras poblaciones europeas, probablemente por la alta salinidad de la laguna murciana.


La nota otorgada fue de Sobresaliente Cum laude. Enhorabuena doctora!.

7ª edición del Curso on-line de Acuicultura

El 11 de enero empezó la 7ª edición del Curso de especialista universitario en acuicultura, que contará con 26 alumnos, 4 más que en la pasada edición.

Los alumnos cursarán 7 materias impartidas por profesores del máster interuniversitario de acuicultura y profesionales del sector.

Las asignaturas que se imparten son las siguientes:

Nutrición en acuicultura,
Reproducción de peces,
Ingeniería de Sistemas Acuícolas,
Diseño de Instalaciones de acuicultura
Patología
Gestión de empresas.


lunes, 18 de enero de 2016

2nd IMPRESS Workshop


A specific website has been created for the 2nd IMPRESS Workshop and 5th AQUAGAMETE Training School in Valencia: 



It contains the detailed program, practical information such as "how to arrive" and a link to register, among others.

5th AQUAGAMETE Training School - 2nd announcement

Dear colleagues,

More detailed information is now available for the 5th AQUAGAMETE Training School which will be held at the Universitat Politècnica de València and PROiSER R+D facilities, Spain.

Please visit: http://trainingschoolvalencia.weebly.com/, where you will find specific and practical information about the event, including a link to the registration form.

We take the chance to remind you that the application deadline is February 5th. Complete applications must be sent via e-mail to aquagamete@gmail.com. Eligible candidates will be announced by February 10th. Please do not register until you have been notified that were selected as trainee. For more detailed information please contact Pepa Bayarri (aquagamete@gmail.com). Applications should include:

• Application form.
• Short Curriculum Vitae in the area.
• Motivation letter for participating in the training school, addressed to Dr. Juan F. Asturiano and indicating the academic/professional status of the applicant.
• Letter of recommendation from the applicant’s supervisor or employer.
• Financial Support justification (brief explanation of expected travel costs derived of the attendance to the Training School, especially in travel, accommodation and meals). 

Please make sure that your name is present in every document.

Financial support from the AQUAGAMETE COST Action (for travelling, accommodation and meals) will be available for selected trainees. The amount of each grant will be calculated upon the information you provided in your “Financial Support justification” document, and taking into account the country of origin.