jueves, 21 de julio de 2016

6th AQUAGAMETE Training School


The 6th AQUAGAMETE COST Action Training School (“Molecular basis of fish gamete quality: genomic tools”) was held in Rennes (France) from June 6th to 10th, 2016, on the Rennes 1 University campus of Beaulieu. It was organized by Dr. Julien Bobe, leader of the AQUAGAMETE Working Group 1. Techniques for evaluation of gametes quality, and Dr. Catherine Labbé. The trainers included 11 staff from the INRA Fish Physiology and Genomics lab, for both the practical and the conferences, and two trainers from Spain, Dr. Paz Herráez and Dr. Francesc Piferrer. The training course welcomed 18 trainees from 11 countries.
The Training School was focused on the Molecular basis of gamete quality and reproduction, with a strong orientation towards genomic tools. The 2h conferences spanned over sperm genetic damage and repair in fish, epigenetics and inheritance, microRNA and female fecundity, and insight into several genomic tools including the sequencing technologies, and transgenesis approaches with a special focus on CrispR/Cas9.
The practical sessions were focused on RNA extraction and quality checking, microarray analysis of fish samples in reproduction research, and sequence searching including tools presentation, primer design and phylogeny/syntheny search.


A visit of the INRA experimental fish facility allowed the trainees to discover the fully equipped behavior room, with the test chambers and the video recording system, and the rearing rooms of more than 7 freshwater species with specific photoperiod, water parameters, and temperature. The functioning of the water recycling system was explained, together with the anti-escapees system required for the transgenic fish of the facility (trout, medaka, goldfish, zebrafish). The dedication of the trainers and the motivation of the trainees ensured the success of this intense week of training.

martes, 19 de julio de 2016

Estancia de Luz Pérez en la Universidad de Utrecht


La Dra. Luz Pérez realizó una estancia a finales de mayo en la Universidad de Utrecht (Campus de Uithof), en el laboratorio del Dr. Rüdiger Schulz (con ella en la foto), con quien ha estado aprendiendo a diferenciar algunos de los estados celulares de los testículos y el modo de cuantificación de estados celulares usado en este laboratorio.
El técnico Hank van der Kant le ha estado enseñando la técnica inmunohistoquímica de BrDU para ver proliferación celular, y ha podido dar un primer vistazo a los cultivos de testículo de zebrafish realizados por Diego Crespo. 

La estancia fue financiada mediante una Short Term Scientific Mission de la Acción COST AQUAGAMETE.

lunes, 18 de julio de 2016

Marina Morini defiende su Tesis Doctoral


El viernes 15 nuestra compañera Marina Morini defendió su Tesis Doctoral titulada: “Molecular approaches related to the European eel (Anguilla anguilla) reproductive process”, que ha sido codirigida por Juan F. Asturiano y David S. Peñaranda, y financiada fundamentalmente por el Proyecto PRO-EEL (UE), el proyecto REPRO-TEMP (MINECO) y la Acción COST AQUAGAMETE.

El Dr. José Miguel Cerdá Reverter (del Instituto de Acuicultura de Torre de la Sal, CSIC) actuó como Presidente del Tribunal, el Dr. Ibon Cancio (de la Universidad del País Vasco/Euskal Herriko Unibertsitatea) fue el Secretario, y la Dra. Salima Aroua (de la Université du Havre, Francia) fue el tercer miembro del mismo.

Al acabar el acto de defensa, el Tribunal le concedió la máxima calificación, Sobresaliente Cum Laude. Enhorabuena Dra. Morini!

El documento final es un compendio de 4 artículos:

Temperature modulates the testis steroidogenesis in European eel
Peñaranda et al., 2016. Comparative Biochemistry and Physiology Part A: Molecular & Integrative Physiology

Expression of nuclear and membrane estrogen receptors in the European eel throughout spermatogenesis
Morini et al., en revisión en Comparative Biochemistry and Physiology Part A: Molecular & Integrative Physiology

Nuclear and membrane progestin receptors in the European eel: characterization and expression in vivo through spermatogenesis
Morini et al., en revisión en PloS ONE

Transcript levels of the soluble sperm factor protein phospholipase C zeta 1 (PLCζ1) increase through induced spermatogenesis in European eel
Morini et al., 2015. Comparative Biochemistry and Physiology Part A: Molecular & Integrative Physiology

con una introducción y una discusión general, y si me lo pedís por e-mail, podemos enviaros un pdf con el texto completo, que estará disponible on-line en unas semanas.


Resumen

The European eel (Anguilla anguilla, L., 1758) population is in dramatic decline, so much so that this species has been listed as “Critically Endangered” on the Red List of Threatened Species, by the International Union for Conservation of Nature (IUCN). The European eel has a complex life cycle, with sexual maturation blocked in the absence of the reproductive oceanic migration, and an inability to mature in captivity without the administration of hormonal treatments. Even though experimental maturation induces gamete production of both sexes, the fertilization results in infertile eggs, unviable embryos and larvae, which die within a few days of hatching. Therefore, understanding the eel reproductive physiology during maturation is very important if we want to recover the wild eel population. Furthermore, due to its phylogenetic position, representative of a basal group of teleosts, the Elopomorphs, the Anguilla species may provide insights into ancestral regulatory physiology processes of reproduction in teleosts, the largest group of vertebrates.
In this thesis, characterization, phylogeny and synteny analyses have given us new insight into the evolutionary history of the reproductive process in vertebrates. The European eel possesses five membrane (mPRs) and two nuclear (nPR or pgrs) progestin receptors. Eel mPRs clustered in two major monophyletic groups. Phylogeny analysis of vertebrate nPRs and PLCζ1 (sperm specific protein) places both eel PLCζ1 and nPR sequences at the base of the teleost clade, which is consistent with the basal position of elopomorphs in the phylogeny of teleosts. To further resolve the origin of the duplicated eel nPRs, synteny analyses of the nPR neighboring genes in several vertebrate genomes were performed. Phylogeny and synteny analyses allowed us to propose the hypothesis that eel duplicated nPRs originated from the 3R.
In order to gain a better understanding of the role of the genes implicated in eel reproduction, analyses of their regulation during experimental maturation were carried out. The change in salinity induced parallel increases in E2 plasma and nuclear estrogen receptor expression levels, revealing a stimulatory effect of salinity on the E2 signalling pathway along the BPG axis, leading to a control of spermatogonial stem cell renewal. Brain and pituitary estrogen receptors may then mediate the stimulation of androgens and steroidogenic enzymes linked to androgen synthesis. Androgen synthesis is not dependent on temperature, but further maturation requires higher temperatures to induce a change in the steroidogenic pathway towards estrogen and progestin synthesis. This is consistent with our studies on estrogen and progestin receptors. In the testis, progestin seems to regulate meiosis through membrane and nuclear progestin receptors, and final sperm maturation seems to be controlled by both estrogen and progestin through the estrogen and progestin membrane receptors. Finally, eel sperm-specific PLCζ1 seems to have an important function in spermatozoa by inducing egg activation and temperature may play a role in its regulation, especially during the process of spermiogenesis.

This thesis attempts to evaluate the physiological function of the genes involved in eel reproduction during spermatogenesis, and demonstrates that salinity and temperature play crucial roles in the sexual maturation of the male European eel.

domingo, 17 de julio de 2016

Estancia de Amin Golpour en la UPV

Amin Golpour Dehsari, etudiante de doctorado de la Faculty of Fisheries and Protection of Waters de la University of South Bohemia (Vodnany, República Checa) ha realizado una estancia con nuestro grupo de investigación desde principios de uno a principios de julio, financiada con una Short Term Scientific Mission de la Acción COST AQUAGAMETE.

Durante estas semanas Amin ha aplicado el protocolo de aislamiento de espermatogonias en el testículo de otras especies de peces, ajustándolo hasta optimizarlo para la anguila europea.
El rendimiento del protocolo se ha evaluado mediante la determinación del número de espermatogonias obtenidas (en la foto) y su porcentaje de viabilidad (>90%).

Esto supone el primer paso para futuros estudios sobre la fisiología de estas células y su utilización en el control de la reproducción de esta especie.


Ha sido estupendo conseguir estos resultados en tan poco tiempo. Gracias Amin!.

martes, 5 de julio de 2016

Nuevas ayudas para estancias cortas de AQUAGAMETE

In the 9th call, the last one in the AQUAGAMETE COST Action, 7 Short Term Scientific Missions were granted: 3 persons from Hungary travelling to the Czech Republic, Slovenia and Spain; 1 participant from Denmark visiting France; 1 person from Portugal travelling to Norway; 1 person from Greece visiting to South Africa and 1 person from Israel going to Norway.


Gook luck to everyone!

Nuestro último artículo, aceptado en Journal of Fish Biology


A comparison of techniques for studying oogenesis in the European eel Anguilla anguilla

Ilaria Mazzeo, Elisabetta Giorgini, Giorgia Gioacchini, Francesca Maradonna, M. Carmen Vílchez, Sylvie Baloche, Sylvie Dufour, Luz Pérez, Oliana Carnevali, Juan F. Asturiano


Abstract

A multi-technique approach was used to study the changes occurring in European eel Anguilla anguilla ovaries during hormonally-induced vitellogenesis. Aside from classic techniques used to monitor the vitellogenic process, such as ovary histology, fat content analysis, sodium dodecyl sulfate–polyacrylamide gel electrophoresis (SDS–PAGE), and vitellogenin enzyme linked immunosorbent assay (ELISA), a new technique, Fourier Transform Infrared (FT-IR) Microspectroscopy, was used for the first time to analyze A. anguilla ovaries. The results from the different techniques provided different ways of approaching the same process. Although it is considered a time consuming approach, of all the employed techniques, histology provided the most direct evidences about vitellogenesis. SDS-PAGE and ELISA were also useful for studying vitellogenesis, whereas fat analysis cannot be used for this purpose. The FT-IR analysis provided a representative IR spectrum for each ovarian stage (PV, EV, MV and LV), demonstrating that it is a valid method able to illustrate the distribution of the oocytes within the ovary slices. The obtained chemical maps confirmed changes in lipid concentrations, and revealed their distribution within the oocytes at different maturational stages. When the results and the accuracy of the FT-IR analysis were compared to those of the traditional techniques commonly used to establish the vitellogenic stage, it became evident that FT-IR is a useful and reliable tool, with many advantages, including the fact that it requires little biological material, the costs involved are low, analysis times are short, and, last but not least, the fact that it offers the possibility of simultaneously analyzing various biocomponents of the same oocyte.

martes, 28 de junio de 2016

Asistimos al VI Congreso Ibérico de Ictiología


Entre el 21 y el 24 de junio se ha desarrollado en Murcia el VI Congreso Ibérico de Ictiología: vocación por los peces de mar, los peces de río y la acuicultura, organizado por la Sociedad Ibérica de Ictiología (SIBIC) y por el grupo de Mar Torralva de la Universidad de Murcia.
En el congreso ha constado de 111 sesiones orales, incluyendo 6 sesiones plenarias, entre las que destacaron la de Ibon Cancio (en la foto) y la de Fernando de la Gándara, y de casi 100 posters. Ha contado con 204 participantes de 15 países diferentes.

Hemos acudido cuatro miembros del Grupo de Acuicultura y Biodiversidad de la UPV (junto a Carina Caldeira en la foto). Miguel Jover abrió la primera sesión de Acuicultura, Endocrinología y Toxicología que se realiza en este Congreso, y que se prolongó durante los tres días del mismo, con una charla titulada: “Situation, prospects and challenges of aquaculture in the Iberian Peninsula”.

Víctor Gallego participó en esta sesión con una comunicación oral titulada: “Sperm quality in fish: factors to consider for application in aquaculture”, mientras que David Sánchez Peñaranda participó con su oral titulada: “Temperature modulates eel maturation”.

Por mi parte, tuve ocasión de participar con una comunicación oral titulada: "Reproduction of European eel in captivity: Where we are?” y en una mesa redonda posterior en la  Sesión Especial: Retos y oportunidades en la investigación de la anguila europea, en la que se hizo un repaso a diversos aspectos relacionados con la realidad de la situación de esta especie en la Península Ibérica y en el resto de Europa.


El Congreso ha sido interesante y variado, y difícilmente superable en cuanto a organización. Muchas gracias a nuestros colegas de Murcia!. Y enhorabuena a la SIBIC, que sigue creciendo.

lunes, 20 de junio de 2016

Nuestro último artículo, aceptado en Comparative Biochemistry and Physiology - Part A: Molecular & Integrative Physiology


Identification of the major proteins present in the seminal plasma of European eel, and how hormonal treatment affects their evolution. Correlation with sperm quality
M. Carmen Vílchez, Davinia Pla, Víctor Gallego, Libia Sanz, Luz Pérez, Juan F. Asturiano, Juan J. Calvete, David S. Peñaranda

Abstract
By first time, 2DE protein profile of European eel seminal plasma has been determined. 14 different proteins corresponding to 9 major families were identified in seminal plasma, through hormonal treatment. Some of them play a part in sperm maturation, including carbonic anhydrase which is responsible for modulating the pH of seminal plasma, and warm temperature acclimation protein, which may play an important role in the final maturation of this species, due to the warm temperature of their spawning ground (in the Sargasso Sea).
Sperm samples were classified into three motility categories depending on the percentage of motile cells, I: 0-25%, II: 25-50% and III: >50%. Different protein profiles were observed depending on the sperm motility categories, specifically, with the apolipoproteins and complement C3. Higher numbers of proteins from the apolipoprotein family were registered at lower motilities; whereas the complement C3-like family was higher in the samples with the highest percentage of motile cells. These results suggest that the proteins linked to the transportation of lipids (apolipoprotein) and to the immune system (complement C3) may carry out their functions at different stages of spermatogenesis. Using SDS-PAGE analysis, 13 bands were identified, most of which migrated between 20 to 60 kDa. In the last weeks of treatment significant increases were observed in the percentage of motile spermatozoa, curvilinear velocity and beat cross frequency. This improvement in sperm quality coincided with a higher amount of proteins located at 19 KDa, therefore, this protein could be involved in sperm motility of the European eel.

Doi:10.1016/j.cbpa.2016.06.025

domingo, 12 de junio de 2016

Nuestro último artículo, aceptado en General and Comparative Endocrinology


Progress, challenges and perspectives on fish gamete cryopreservation: A mini-review

Juan F. Asturiano, Elsa Cabrita, Ákos Horváth

Abstract
Protocols for the cryopreservation of fish gametes have been developed for many different fish species, in special, freshwater salmonids and cyprinids. Methods for sperm freezing have progressed during the last decades due to the increasing number of potential applications: aquaculture (genetic improvement programs, broodstock management, helping with species having reproductive problems), biotechnology studies using model fish species (preservation of transgenic or mutant lines), cryobanking of genetic resources from endangered species, etc.
This mini-review tries to give an overview of the present situation of this area of research, identifying the main challenges and perspectives, redirecting the reader to more in-depth reviews and papers.

martes, 7 de junio de 2016

Manifiesto “Ríos sin barreras”


Por iniciativa de la Sociedad Ibérica de Ictiología (SIBIC)  en colaboración con la Asociación para el Estudio y Mejora de los Salmónidos (AEMS – Ríos con Vida), la Asociación Ibérica de Limnologia (AIL), el Centro Ibérico de Restauración Fluvial (CIREF), la Sociedad Española de Ornitología (SEO/Birdlife), la World Fish Migration Foundation (WFMF) y el World Wildlife Found (WWF), se ha enviado a los medios de comunicación el manifiesto Ríos sin barreras que podeis encontrar en la web de la SIBIC: http://www.sibic.org/manifiesto-rios-sin-barreras/

Os pedimos a todos que nos ayudéis a darle la máxima difusión! 

viernes, 3 de junio de 2016

Announcement 7th AQUAGAMETE Training School (Fish proteomics)

The AQUAGAMETE COST Action (aquagamete.webs.upv.es/web) is pleased to announce the 7th AQUAGAMETE Training School.

The 7th AQUAGAMETE Training School is aimed towards young researchers (PhD students, young post-docs and MSc students) as well as representatives from industry. The course will be focused on Fish proteomics and will include theoretical and practical classes. The course will provide an introduction to the field of proteomics and emphasizes hands-on experience with two dimensional electrophoresis and mass spectrometry protein identification.

Specific topics include: Introduction to proteomics; Proteomics in reproductive science; Proteomics in fish research; Techniques of sample preparation for proteomic analysis; Two dimensional electrophoresis; Detection of spots, digestion and protein identification; Bioinformatics in proteomic research

The 7th AQUAGAMETE Training School will highlight the most recent developments in each topic, promoting a strong interaction between experienced trainers working in different proteomic areas and trainees.

The course will take place between 26 - 30th September 2016 at the Institute of Animal Reproduction and Food Research in Olsztyn, Poland.

The number of participants will be restricted to 9 persons. Financial support from the AQUAGAMETE COST Action (for travelling, meals and accommodation) will be available for trainees. Registration will be available from 1st August and both for students (no registration fee) and for industry representatives (fee of 300 euros) and includes access to all lectures and laboratorial practices, support material and transport between facilities and main hotel.

The application deadline is 10th July and complete applications must be sent via e-mail to aquagamete@gmail.com. Eligible candidates will be announced by the end of July.

For more detailed information please contact Andrzej Ciereszko (a.ciereszko@pan.olsztyn.pl).

Applications should include:
  • Application form
  • short Curriculum Vitae in the area (only for students)
  • motivation letter for participating in the training school, which should indicate the academic/professional status of the applicant
  • letter of recommendation from the applicant’s supervisor or employer
  • Financial support justification where candidates should estimate their costs in travelling, accommodation and meals during their attendance to the Training School.

We would appreciate if you could forward this announcement via e-mail to colleagues or students who may be interested in participating. More information available soon at:  http://aquagamete.webs.upv.es/

jueves, 2 de junio de 2016

Celebramos el Día Mundial de la Migración de los Peces

El pasado sábado 21 de mayo el Grupo de Acuicultura y Biodiversidad (GAB) de la Universitat Politècnica de València celebró el Día Mundial de la Migración de los Peces (World Fish Migration Day) en el Centro de Interpretación Racó de l’Olla, en el Parque Natural de La Albufera de Valencia. Este evento se realizó en el marco de la Red de Formación Europea (European Training Network) IMPRESS (Marie Sklodowska-Curie Actions, H2020), en la que participamos.

El objetivo del World Fish Migration Day  es concienciar a la población de la importancia de los ríos en la migración de los peces, que deberían poder nadar por ellos sin encontrar obstáculos como presas o azudes construidos por el hombre que impiden sus movimientos migratorios. La primera edición tuvo lugar en mayo de 2014 y en ella se organizaron un total de 270 eventos en todo el mundo. Sin embargo, en esta segunda edición la cifra ha alcanzado los 450 eventos en 61 países, registrándose unos 15 millones de seguidores en las redes sociales.

Cerca de 240 personas visitaron el Centro de Interpretación el día 21 a lo largo de la mañana, y disfrutaron de las actividades organizadas. En primer lugar, tuvieron la oportunidad de escuchar las charlas informativas a cargo del Dr. Asturiano, quien presentó al público el World Fish Migration Day y sus objetivos, el ciclo reproductivo de la anguila europea y sus problemas en acuicultura, cómo el GAB trata de ayudar con sus experimentos en la UPV y cómo el proyecto IMPRESS trata asimismo de ayudar a otras especies migradoras como el salmón atlántico y los esturiones, muchos de ellos en peligro de extinción.


Los visitantes pudieron ver ejemplares adultos (machos y hembras) de anguila, así como angulas.



Después de cada charla, la cuentacuentos Lorena Comín, de Disparatario, narró la historia amenizada con juegos de “Ann Guillidae”, escrita por Eva Lucía Bayarri e ilustrada por Susana Vilanova. La historia cuenta cómo una pequeña angula (Ann) llega a la Albufera y conoce a un samaruc (Valencia hispanica), Sam. Ann habla a Sam sobre su largo viaje desde el Mar de los Sargazos y sobre su intención de quedarse allí, en agua dulce, hasta que vuelva a partir hacia el lugar donde nació. Así, de una forma didáctica, los jóvenes asistentes aprendieron cómo es el ciclo vital de la anguila europea.




Los niños pudieron colorear divertidos dibujos de peces migradores, la mayoría de ellos proporcionados por la plataforma de World Fish Migration Day, y opcionalmente, participar en su concurso de dibujo.

Finalmente, pudieron visitar uno de los sitios por donde las anguilas entran y salen de la Albufera al mar, la Gola de El Pujol. El Taller de Empleo Conservación del Bosque Devesa Albufera II del Ayuntamiento de Valencia colaboró realizando visitas guiadas a lo largo de la “Gola de El Pujol”, durante las cuales explicaron a los visitantes detalles interesantes sobre la Albufera, la pesca tradicional de angulas, las aves que se pueden avistar, las compuertas que regulan el nivel del agua de la Albufera en función de las necesidades del cultivo de arroz, etc.
En general, los visitantes disfrutaron de este evento, y muchos de ellos reconocieron que no sabían que la anguila que tradicionalmente se había estado utilizando en la gastronomía local desde hace tantísimo tiempo era una criatura tan excepcional.

Nuestro agradecimiento al personal del Centro de Visitantes Racó de l´Olla, a los miembros del Taller de Empleo Conservación del Bosque Devesa Albufera II del Ayuntamiento de Valencia, a la Cofradía de Pescadores de El Palmar, a la Generalitat Valenciana y a la ETN IMPRESS.

lunes, 23 de mayo de 2016

Nuestro último artículo, aceptado en General and Comparative Endocrinology

Development of sperm vitrification protocols for freshwater fish (Eurasian perch, Perca fluviatilis) and marine fish (European eel, Anguilla anguilla)

Eszter Kása, Gergely Bernáth, Tímea Kollár, Daniel Żarski, Jelena Lujić, Zoran Marinović, Zoltán Bokor, Árpád Hegyi, Béla Urbányi, M. Carmen Vílchez, Marina Morini, David S. Peñaranda, Luz Pérez, Juan F. Asturiano, Ákos Horváth

Abstract
Vitrification was successfully applied to the sperm of two fish species, the freshwater Eurasian perch (Perca fluviatilis) and marine European eel (Anguilla anguilla). Sperm was collected, diluted in species-specific non-activating media and cryoprotectants and vitrified by plunging directly into liquid nitrogen without pre-cooling in its vapor. Progressive motility of fresh and vitrified-thawed sperm was evaluated with computer-assisted sperm analysis (CASA). Additional sperm quality parameters such as sperm head morphometry parameters (in case of European eel) and fertilizing capacity (in case of Eurasian perch) were carried out to test the effectiveness of vitrification. The vitrification method for Eurasian perch sperm resulting the highest post-thaw motility (14 ± 1.6%) was as follows: 1:5 dilution ratio, Tanaka extender, 30% cryoprotectant (15% methanol + 15% propylene-glycol), cooling device: Cryotop, 2 μl droplets, and for European eel sperm: dilution ratio 1:1, with 40% cryoprotectant (20% MeOH and 20% PG), and 10% FBS, cooling device: Cryotop, with 2 μl of sperm suspension. Viable embryos were produced by fertilization with vitrified Eurasian perch sperm (neurulation: 2.54±1.67%). According to the ASMA analysis, no significant decrease in head area and perimeter of vitrified European eel spermatozoa were found when compared to fresh spermatozoa.

Doi:10.1016/j.ygcen.2016.05.010


martes, 3 de mayo de 2016

Día Mundial de la Migración de los Peces (World Fish Migration Day)


El próximo 21 de mayo se celebrará en el Centro de Interpretación Racó de l’Olla, en el Palmar, una jornada de divulgación sobre la anguila europea. Esta actividad se realiza en el marco del Día Mundial de los Peces Migradores (World Fish Migration Day, www.worldfishmigrationday.com) y de la Acción Europea Marie Sklodowska-Curie IMPRESS (http://www.impress-itn.eu/). Se trata de un acontecimiento internacional en el que se  desarrollarán cerca de 300 eventos en todo el mundo, para concienciar a la población sobre la situación de los peces migradores y de nuestros ríos y lagos, su papel ecológico y gran importancia. En mayo de 2014 tuvo lugar la primera edición, y en esta segunda se espera superar las cifras de participación.
El Grupo de Acuicultura y Biodiversidad de la Universitat Politècnica de València, especializado en el estudio de la reproducción de esta especie (http://acuiculturaenvalencia.blogspot.com.es/), ha organizado actividades, dirigidas tanto a grandes como a pequeños, que consistirán en:
- Charlas para dar a conocer las peculiaridades del ciclo vital de la anguila (a las 10:00, 11:00, 12:00 y 13:00);
- Exposición de ejemplares vivos y artes de pesca locales;
- Concurso de dibujo y coloreado con la temática de los peces migradores (a cargo de la plataforma World Fish Migration Day);
- Narración del cuento “Ann Guillidae”, escrito por Eva Lucía Bayarri e ilustrado por Susana Vilanova, a cargo de la Cuentacuentos Lorena Comín (pases a las 10:20, 11:20, 12:20 y 13:20);

- Visitas guiadas por el Itinerario de la Gola de El Pujol, uno de los sitios por donde la anguila entra y sale de la Albufera, en colaboración con el servicio Devesa-Albufera del Ayuntamiento de Valencia (a las 11:00, 12:00 y 13:00).



jueves, 21 de abril de 2016

Nuestro último artículo, aceptado en Aquaculture

Hormonal manipulations for the enhancement of sperm production in cultured fish and evaluation of sperm quality

Constantinos C. Mylonas, Neil J. Duncan, Juan F. Asturiano

Abstract
This article reviews the use of hormonal treatments to enhance sperm production in aquaculture fish and the methods available for evaluating sperm quality. The different types of testis development are examined and a brief review is presented of the endocrine regulation of spermatogenesis in fishes, including the increasing evidence of the existence of spermatozoa subpopulations. Hormonal manipulations are employed to induce spermatogenesis in species such as the freshwater eels, to synchronize maximal sperm volume to ovulation for in vitro fertilization and to enhance sperm production in species with poor spermiation. The hormones that are employed include gonadotropins (GtH) of piscine or mammalian origin, and gonadotropin-releasing hormone agonists (GnRHa) administered by injections or controlled-release delivery systems, with or without dopaminergic inhibitors.
Pheromones in the culture water and hormones added to the sperm in vitro have also been employed to enhance spermiation and sperm quality, respectively, in some fishes. Hormonal therapies usually do not affect sperm quality parameters, except in cases where fish fail to spermiate naturally or produce very small volumes of high-density sperm. Different parameters have been used to evaluate fish sperm quality, including sperm volume and density, spermatozoa motility and morphometry, and seminal plasma composition. The development of Computer-Assisted Sperm Analysis (CASA) systems made possible the estimation of a higher number of sperm motion parameters using an objective, sensitive and accurate technique. The development of Assisted Sperm Morphology Analysis (ASMA) software has introduced a new approach for sperm evaluation studies, demonstrating changes in the spermatozoa related to reproductive season, hormonal treatments or the cryopreservation processes, and how these may be related to changes in sperm motility and fertilization capacity. The article concludes with a few practical protocols for the enhancement of sperm production in aquaculture species.

Available here: http://authors.elsevier.com/sd/article/S004484861630206X

miércoles, 13 de abril de 2016

Nuestro último artículo, aceptado en Reproduction in Domestic Animals


First production of larvae using cryopreserved sperm: Effects of preservation temperature and cryopreservation on European eel sperm fertilization capacity

Juan F. Asturiano, Sune R. Sørensen, Luz Pérez, Peter Lauesen, Jonna Tomkiewicz

Abstract

Sperm cryopreservation is a useful tool in captive fish reproduction management, i.e. in order to synchronize gamete production, especially in the case of species as the European eel, where the time of female spawning readiness is unpredictable. Several protocols to cryopreserve sperm of this species have been described, but until recently fertilization trials were not feasible. The present study evaluated the effect of cold storage of diluted sperm prior to fertilizations and tested if a previously defined protocol for European eel sperm cryopreservation can be successfully applied in fertilization trials to produce viable offspring. In our experiment, the sperm motility was evaluated after the extraction and the best samples were selected and pooled. Until stripping of eggs and fertilization, diluted sperm samples were maintained at either 4 or 20 ºC, or cryopreserved, following existing protocols. Fertilization of two egg batches was attempted. Diluted sperm caused a similar percentage of fertilized eggs and a similar number of embryos and larvae, independently of storage temperature (4 or 20 ºC). The cryopreserved sperm resulted in a lower percentage of fertilized eggs, but embryos developed and a few larvae (“cryolarvae”) were obtained 55 h after fertilization in one of the two egg batches. This result evidences that the tested cryopreservation protocol is applicable for eel reproduction management, although improvements will be required to enhance fertilization success.

Oferta de contrato predoctoral en Berlín dentro del Proyecto IMPRESS

The Leibniz-Institute of Freshwater Ecology and Inland Fisheries (www.igb-berlin.de) is the largest research institute for freshwater research in Germany. It is member of the Leibniz Association and the Forschungsverbund Berlin e.V. IGB has close links to all three universities in the German capital and currently hosts about 50 doctoral students from approximately 15 different nations. Since 1996, the IGB has been committed to research on sturgeon remediation and restoration in the tributaries of the Baltics and the North Sea, including several national and international projects and programmes. Within the EU Marie Sklodowska-Curie Initial Training Network IMPRESS (Improved production strategies for endangered freshwater species, http://www.impress-itn.eu), the Research Group Applied Fish Physiology and Aquaculture led by Dr. Sven Würtz is looking for a hard-working, enthusiastic
Early-stage researcher
Trait-related optimization of hatchery technology to improve fitness and stocking efficiency in sturgeon
This is position 7 within IMPRESS, available from now on until 31.08.2018

IMPRESS aims at developing new technologies for improved production, management and conservation of threatened iconic diadromous fish species. IMPRESS is set up as intersectoral network of 15 ESR hosted by 9 different hosts covering molecular biology, physiology, and aquaculture technology, as well as human dimensions of biodiversity conservation. The project will focus on assessing and improving fitness of hatchery-reared early life stages in the wild. Challenge tests will be carried out to characterize ecophysiological adaptations to fluctuating environmental challenges and improve aquaculture technologies in conservation aquaculture. During the project the ESR will spend up to 6 months at the Norwegian University of Life Sciences (host Ian Meyer) assessing impaired cognitive ability and behaviour in hatchery of sturgeon. Finally the student will carry out transcriptome analysis of selected samples at the ZF Screens (Ron Dirks) in Leiden, Netherlands. This mobility will enhance multidisciplinary training of the student, and give him/her important experience of both academic and non-academic sectors (training school programme).
More details on the topic, the ITN network, the location, the team of supervisors and their groups, the expected mobility, salary and general eligibility criteria can be found in a long version of this job advertisement posted at www.igb-berlin.de/job-offers.html.
Applicants should have a 2.1 degree or equivalent in a relevant life science (biology, fisheries sciences, aquaculture and environmental sciences). Excellent communication skills in English are required. Experience with molecular techniques (e.g. qPCR, transcriptome analysis) and analytical chemistry (mass spectrometry, chromatography) is highly preferred. Candidates with a background in aquaculture (particularly larval rearing) will be deemed highly competitive.
According to EU regulations candidate students must not have resided for more than 12 months during the previous 3 years in the prospective host country, Germany.
Salary is paid according to the regulations of the programme. In keeping with the IGB's policy regarding gender equity, female applicants are particularly encouraged. Among candidates of equal aptitude and qualifications, a person with disabilities will be given preference.

Review of applications meeting the required standards will start immediately and continue until a candidate is appointed. Please send your application and further inquiries to Dr. Sven Würtz (wuertz@igb-berlin.de).

La SIBIC estrena nueva web


La Sociedad Ibérica de Ictiología cumplió 5 años el pasado diciembre de 2015 y ha renovado su web (www.sibic.org). La nueva web es mucho más moderna y dinámica. Se ha construido para que sea totalmente accesible desde ordenadores, tablets y smartphones. También se han integrado la web de la revista Fishes in Mediterranean Environments (www.fishmedjournal.org) y los congresos celebrados hasta el momento por la SIBIC. Además se ha añadido la versión en inglés para facilitar y fomentar la visibilidad y las relaciones internacionales.
Os invitamos a navegar por la nueva sibic.org


martes, 12 de abril de 2016

Nuestro último artículo, aceptado en Comparative Biochemistry and Physiology - Part A: Molecular & Integrative Physiology


Sodium affects the sperm motility in the European eel

M. Carmen Vílchez, Marina Morini, David S. Peñaranda, Víctor Gallego, Juan F. Asturiano, Luz Pérez

Abstract

The role of seminal plasma sodium and activation media sodium on sperm motility was examined by selectively removing the element from these two media, in European eel sperm. Sperm size (sperm head area) was also measured using an ASMA (Automated Sperm Morphometry Analyses) system, in the different conditions. Intracellular sodium [Na+]i was quantitatively analyzed by first time in the spermatozoa from a marine fish species. Measurement of [Na+]i was done before and after motility activation, by Flow Cytometry, using CoroNa Green AM as a dye. Sperm motility activation induced an increase in [Na+]i, from 96.72 mM in quiescent stage to 152.21 mM post-activation in seawater. A significant decrease in sperm head area was observed post-activation in seawater. There was a notable reduction in sperm motility when sodium was removed from the seminal plasma, but not when it was removed from the activation media. Sodium removal was also linked to a significant reduction in sperm head area in comparison to the controls. Our results indicate that the presence of the ion Na+ in the seminal plasma (or in the extender medium) is necessary for the preservation of sperm motility in European eel, probably because it plays a role in maintaining an appropriate sperm cell volume in the quiescent stage of the spermatozoa.

martes, 5 de abril de 2016

Nueva Training School de AQUAGAMETE

The AQUAGAMETE COST ACTION is pleased to announce the 6th AQUAGAMETE training school which will be focused on the Molecular basis of gamete quality and reproduction, with a strong orientation towards GENOMIC TOOLS

The course will be organized by the Fish Physiology and Genomics Department of INRA, Rennes (France) (coordination by Julien Bobe and Catherine Labbé), and will be held in Rennes from June 6th to 10th 2016.

The course will include conferences on:
- Sperm genetic damage and repair in Fish (Paz Herraez, Spain)
- Overview of sequencing technologies and strategies available to analyze fish reproduction (Yann Guiguen, France)
- Epigenetics and inheritance (Francesc Piferrer, Spain)
- Transgenesis approaches in fish reproduction (speaker to be determined)
- CrispR/Cas9 technology for research on fish reproduction (Amaury Herpin, France)
- microRNA and female fecundity (Amine Bouchareb, France)

The practical sessions will be coordinated by the Fish Physiology and Genomics staff.  Include experiment on: 
- RNA extraction (2h30)
- Microarray analysis of fish samples in reproduction research (10h)
- Sequence searching including tools presentation, primer design, phylogeny/syntheny search (5 h)

The number of participant is restricted to 16 persons. Priority will be given to early stage researchers (MSc students, PhDstudents, young post-docs) and to participants with no experience in genomics and with a professional project related to gamete quality or fish reproduction, including representative from industry.

Financial support from the AQUAGAMETE COST action for travelling, accommodation and meals will be available for students. The access to lectures, laboratory facilities and support material is supported for all participants by the AQUAGAMETE COST action and by the host Fish Physiology and Genomics department.

The application deadline is May 2nd, 2016. Complete applications must be sent via e-mail to aquagamete@gmail.com. Eligible candidates will be announced by May 6th. For more detailed information please contact Pepa Bayarri (aquagamete@gmail.com).

Applications should include the following documents:
• Application form (attached);
• Short Curriculum Vitae in the area;
• Motivation letter for participating in the training school, which should indicate the academic/professional status of the applicant;
• Letter of recommendation from the applicant’s supervisor or employer;
• Financial Support justification (only for students).

More information available soon at: http://aquagamete.webs.upv.es