miércoles, 27 de mayo de 2015

Nuestro último artículo, aceptado en Comparative Biochemistry and Physiology

Transcript levels of the soluble sperm factor protein phospholipase C zeta 1 (PLCζ1) increase through induced spermatogenesis in European eel

M. Morini, D.S. Peñaranda, M.C. Vílchez, V. Gallego, R. Nourizadeh-Lillabadi, J.F. Asturiano, F.-A. Weltzien, L. Pérez

Activation at fertilization of the vertebrate egg is triggered by Ca2+ waves. Recent studies suggest the phospholipase C zeta (PLCζ), a sperm-specific protein, triggers egg activation by an IP3-mediated Ca2+ release and allow Ca2+ waves at fertilization.
In the present study we cloned, characterized, and phylogenetically positioned the European eel PLCζ (PLCζ1). It is 1521bp long, with 10 exons encoding an open reading frame of 506 amino acids. The amino acid sequence contains an EF-hand domain, X and Y catalytic domains, and a carboxy-terminal C2 domain, all typical of other PLCζ orthologous. The sequence is truncated not only at the N-terminus of the EF-hand domain, as in all teleost PLCζ, but also in the C-terminal region of the X-domain and in a large part of the N-terminal X/Y linker region.

The tissue distribution was studied, and the gene expression was determined in testis during induced sexual maturation at three different thermal regimes. Also, brain and pituitary expression were studied through sex maturation at constant temperature. plcζ1 was expressed in brain of male and female, in testis but not in ovaries. By first time in vertebrates, it is reported plcζ1 expression in the pituitary gland. Testis plcζ1 expression increased through spermatogenesis under all the thermal regimes, but being significantly elevated at lower temperatures. It was very low when testis contained only spermatogonia or spermatocytes, while maximum expression was found during spermiogenesis. These results support the hypothesis for an eel sperm-specific PLCζ1 inducing egg activation, similarly to mammals and some teleosts, but different from some other teleost species, which express this protein in ovaries, but not in testes.

jueves, 21 de mayo de 2015

Se abre el plazo de matrícula del Máster Interuniversitario en Acuicultura

El Máster Oficial Interuniversitario en Acuicultura es un programa de postgrado para graduados que busquen profesionalizarse o desarrollar un doctorado en el sector acuícola. Está organizado conjuntamente por las dos grandes universidades públicas de Valencia (UV y UPV), para poder ofrecer al alumno los mejores expertos de ambas universidades y así proporcionarles las herramientas necesarias para el éxito profesional. Cuenta con la colaboración del Instituto de Acuicultura de Torre de la Sal, así como con apoyos de diversas empresas de acuicultura.
Más info:
masteracuicultura@posgrado.upv.es

martes, 19 de mayo de 2015

Víctor Gallego consigue el Premio Extraordinario de Tesis Doctoral


La UPV ha concedido el Premio Extraordinario de Tesis Doctoral (convocatoria 2015) a nuestro compañero Víctor Gallego por la Tesis titulada: Sperm physiology and quality in two marine teleosts: Anguilla anguilla & Takifugu niphoblesque defendió el 2 de diciembre de 2013 y con la que había conseguido un Sobresaliente cum laude.
Además de la propia memoria, la selección se ha basado en el número total de artículos, capítulos de libro, comunicaciones en congresos y estancias realizadas durante su doctorado.

Enhorabuena Dr. Gallego!