Publicaciones (2008-24)

2024

Characterization of potential spermatogonia biomarker genes in the European eel (Anguilla anguilla)
M. Blanes-García, Z. Marinović, J.G. Herranz-Jusdado, X. Xie, L. Ferrão, V. Gallego, L. Pérez, A.R. Baloch, Á. Horváth, M. Pšenička, J.F. Asturiano, M. Morini
Fish Physiology and Biochemistry
doi: 10.1007/s10695-024-01338-1

Successful cryopreservation in biodegradable containers of sperm from aquaculture Mediterranean fishes
T.S. França, W.A. González-López, M.P. Sanchez, L. Ferrão, L.P. Borges, A. Belenguer, P.G. Holhorea, J.C. Calduch-Giner, D.P. Streit Jr, A. Felip, A. Gómez, J. Pérez-Sánchez, J.F. Asturiano
Theriogenology 216: 53-61
doi: 10.1016/j.theriogenology.2023.12.016

Superoxidase dismutases (SODs) in the European eel: gene characterization, expression response to temperature combined with hormonal maturation and possible migratory implications

L. Ferrão, M. Blanes-García, L. Pérez, J.F. Asturiano, M. Morini

Comparative Biochemistry and Physiology - Part A - Molecular & Integrative Physiology 290:111590

doi: 10.1016/j.cbpa.2024.111590

Effect of seawater temperature and pH on the sperm motility of the European eel

M.P. Sanchez, T.S. França, W.A. González-López, M. Morini, J.F. Asturiano, L. Pérez

Fish Physiology and Biochemistry

doi: 10.1007/s10695-024-01311-y

Xenotransplantation of European eel (Anguilla anguilla) spermatogonia in zebrafish (Danio rerio) and European sea bass (Dicentrarchus labrax)

M. Blanes-García, Z. Marinović, M. Morini, Á. Horváth, A. Vergnet, J.F. Asturiano 

Fishes 

doi: 10.3390/fishes9070290

Effects of cold seawater pre-treatments on the spermatogenesis process and the reproductive performance of male European eels

L. Ferrão, M. Morini, W.A. González-López, V. Gallego, A. Felip, L. Pérez, J.F. Asturiano

Fish Physiology and Biochemistry

doi: 10.1007/s10695-024-01402-w

2023

Silver nanoparticles and silver ions indistinguishably decrease sperm motility in Pacific oysters (Magallana gigas) after short-term direct exposure

F. Fernández-García, A. Carvalhais, A. Marques, I.B. Oliveira, S. Guilherme, H. Oliveira, C.C.V. Oliveira, E. Cabrita, J.F. Asturiano, M. Pacheco, C. Mieiro

Environmental Toxicology and Pharmacology 101: 104202

doi: 10.1016/j.etap.2023.104202

2022

Technologies and strategies for ex situ conservation of aquatic organisms: the role of cryopreservation in long-term management 

E. Cabrita, Á. Horváth, Z. Marinović, J.F. Asturiano

In: Cellular and Molecular Approaches in Fish Biology

I. Fernández and J. Fernandes (eds.). Chapter 1, Pp: 1-48.

Editorial: Elsevier

eBook ISBN: 9780128222744; Paperback ISBN: 9780128222737

doi: 10.1016/B978-0-12-822273-7.00011-2

Sex on the beach

J.F. Asturiano, V. Gallego

Frontiers in Ecology and the Environment (EcoPics section) 20(1): 30.

doi: 10.1002/fee.2460

Using osmotic pumps to induce the production of gametes in male and female European eels

M. Blanes-García, P. García-Salinas, M. Morini, L. Pérez, J.F. Asturiano, V. Gallego

Animals 12: 387

doi: 10.3390/ani12030387

Characterisation of European eel (Anguilla anguilla) spermatozoa morphometry using Trumorph tool in fixed and non-fixed samples
C. Caldeira, S. Hernández-Ibánez, A. Vendrell, A. Valverde, A. García-Molina, V. Gallego, J.F. Asturiano, C. Soler
Aquaculture 553: 738047
doi: 10.1016/j.aquaculture.2022.738047

Ex vivo exposure to titanium dioxide and silver nanoparticles mildly affect sperm of gilthead seabream (Sparus aurata) - A multiparameter spermiotoxicity approach

A. Carvalhais, I.B. Oliveira, H. Oliveira, C.C.V. Oliveira, L. Ferrão, E. Cabrita, J.F. Asturiano, S. Guilherme, M. Pacheco, C.L. Mieiro

Marine Pollution Bulletin 177: 113487
doi: 10.1016/j.marpolbul.2022.113487 

 

 

Ionic control of sperm motility and trials for the improvement of pufferfish (Takifugu alboplumbeus) sperm extenders 

L. Pérez, J.F. Asturiano, M. Yoshida, V. Gallego 

Aquaculture 554: 738146 

doi: 10.1016/j.aquaculture.2022.738146

Dynamic evolution of transient receptor potential vanilloid (TRPV) ion channel family with numerous gene duplications and losses

M. Morini, C. Bergqvist, J.F. Asturiano, D. Larhammar, S. Dufour

Frontiers in Endocrinology 13:1013868 (23 pp) (2022)

doi: 10.3389/fendo.2022.1013868

 

2021

Milt production and sperm quality in European eel (Anguilla anguilla) in relation to hormonal treatment

P. Koumpiadis, D.E. Sganga, S.N. Politis, V. Gallego, I.A.E. Butts, J.F. Asturiano, I.E. Batjakas, J. Tomkiewicz

Reproduction and Domestic Animals 56(12): 1497-1505

doi: 10.1111/rda.14011

Reproductive Anatomy of Chondrichthyans: Notes on Specimen Handling and Sperm Extraction. II. Sharks and Chimaeras

P. García-Salinas, V. Gallego, J.F. Asturiano

Animals 11: 2191. (2021)

doi: 10.3390/ani11082191

Development of Sperm Cryopreservation Protocols for Sharks and Rays: New Tools for Elasmobranch Conservation 

P. García-Salinas, V. Gallego, J.F. Asturiano

Frontiers in Marine Science 8: 689089

doi: 10.3389/fmars.2021.689089

Reproductive Anatomy of Chondrichthyans: Notes on Specimen Handling and Sperm Extraction. I. Rays and Skates

P. García-Salinas, V. Gallego, J.F. Asturiano

Animals 11(7): 1888. (2021)

doi: 10.3390/ani11071888

Sperm quality parameters of Iberian toothcarp (Aphanius iberus) and Valencia toothcarp (Valencia hispanica): new conservation tools from a gamete perspective

M. Blanes, P. Risueño, L. Pérez, J.F. Asturiano, V. Gallego

Aquaculture, 530: 735819 (2021)

doi: 10.1016/j.aquaculture.2020.735819

2020

Dietary amino acids impact sperm performance traits for a catadromous fish, Anguilla anguilla reared in captivity

I.A.E. Butts, G.S. Hilmarsdóttir, V. Zadmajid, V. Gallego, L.K. Holst, J. Støttrup, C. Jacobsen, M. Krüger-Johnsen, S. Politis, J.F. Asturiano, J. Tomkiewicz

Aquaculture, 518: 734602
Doi: 10.1016/j.aquaculture.2019.734602

Identification and stable expression of vitellogenin receptor (VTGR) through vitellogenesis in the European eel 

M. Morini, A.G. Lafont, G. Maugars, S. Baloche, S. Dufour, J.F. Asturiano, L. Pérez 
Animal: The International Journal of Animal Biosciences, 14(6): 1213-1222 
Doi: 10.1017/S1751731119003355

Intracellular pH regulation and sperm motility in the European eel 
L. Pérez, V. Gallego, J.F. Asturiano
Theriogenology, 145: 48-58 
doi: 10.1016/j.theriogenology.2020.01.026
Disponible aquí: https://authors.elsevier.com/c/1aSZy,28Lh5bqd

Libro: Reproduction in Aquatic Animals. From Basic Biology to Aquaculture Technology

Editores: M. Yoshida (University of Tokyo), J.F. Asturiano (UPV).

Editorial: Springer Nature

2020. 379 páginas    

ISBN: 978-9811522895; ISBN 978-981-15-2290-1 (eBook)

doi: 10.1007/978-981-15-2290-1

Fish sperm maturation, capacitation, and motility activation

L.M. Pérez (2020).

In: Reproduction in Aquatic Animals. From Basic Biology to Aquaculture Technology. M. Yoshida and J.F. Asturiano (eds.). Chapter 4, Pp: 47-67. ISBN: 978-981-15-2290-1.

Improvements on the reproductive control of the European eel

J.F. Asturiano (2020)

In: Reproduction in Aquatic Animals. From Basic Biology to Aquaculture Technology. M. Yoshida and J.F. Asturiano (eds.). Chapter 14, Pp: 293-320. ISBN: 978-981-15-2290-1.

2019

Fish sperm motility assessment as a tool for aquaculture research, a historical approach

V. Gallego, J.F. Asturiano

Reviews in Aquaculture, 11: 697-724
Doi: 10.1111/raq.12253

Recombinant vs purified mammal gonadotropins as maturation hormonal treatments of European eel males

J.G. Herranz-Jusdado, C. Rozenfeld, M. Morini, L. Pérez, J.F. Asturiano, V. Gallego.
Aquaculture, 501: 527-536
Doi: 10.1016/j.aquaculture.2018.12.015

Standardization of sperm motility analysis by using CASA-Mot for Atlantic salmon (Salmo salar), European eel (Anguilla anguilla) and Siberian sturgeon (Acipenser baerii).

C. Caldeira, S. Hernández, A. Valverde, P. Martin, J.G. Herranz-Jusdado, V. Gallego, J.F. Asturiano, B. Dzyuba, M. Psenicka, C. Soler.

Aquaculture, 502: 223-231
Doi: 10.1016/j.aquaculture.2018.12.001

Comparison of European eel sperm cryopreservation protocols with standardization as a target 
J.G. Herranz-Jusdado, V. Gallego, M. Morini, C. Rozenfeld, L. Pérez, E. Kása, T. Kollár, A. Depincé, C. Labbé, Á. Horváth, J.F. Asturiano
Aquaculture, 498: 539-544
Doi: 10.1016/j.aquaculture.2018.09.006

European eel sperm storage: optimization of short-term protocols and cryopreservation of large volumes

J.G. Herranz-Jusdado, V. Gallego, C. Rozenfeld, M. Morini, L. Pérez, J.F. Asturiano

Aquaculture, 506: 42-50
Doi: 10.1016/j.aquaculture.2019.03.019

Sperm handling in aquatic animals for artificial reproduction

J. Beirao, M. Boulais, V. Gallego, J.K. O'Brien, S. Peixoto, T.R. Robeck, E. Cabrita

Theriogenology, 133: 161-178. 2019

Doi: 10.1016/j.theriogenology.2019.05.004

Eel sperm cryopreservation: an overview

J.G. Herranz-Jusdado, V. Gallego, M. Morini, C. Rozenfeld, L. Pérez, T. Müller, Á. Horváth, H. Ohta, J.F. Asturiano

Theriogenology, 133: 210-215
Doi:10.1016/j.theriogenology.2019.03.033
 
de novo European eel transcriptome provides insights into the evolutionary history of duplicated genes in teleost lineages 
C. Rozenfeld, J. Blanca, V. Gallego, V. García-Carpintero, J.G. Herranz-Jusdado, L. Pérez, J.F. Asturiano, J. Cañizares, D.S. Peñaranda 
PLoS ONE 14(6): e0218085. 2019
Doi: 10.1371/journal.pone.0218085
Disponible (Open Access): https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0218085

Cold seawater induces early maturational stages in the BPG axis of European eel males 

C Rozenfeld, V. García-Carpintero, L. Pérez, V. Gallego, J.G. Herranz-Jusdado, H. Tveiten, H.K. Johnsen, R. Fontaine, F.A. Weltzien, J. Cañizares, J.F. Asturiano, D.S. Peñaranda 
BMC Genomics (2019) 20:597
Doi: 10.1186/s12864-019-5969-6
Disponible (Open Access): https://rdcu.be/bLorR

2018 

Subjective and objective assessment of fish sperm motility: when the technique and technicians matter

V. Gallego, J.G. Herranz-Jusdado, C. Rozenfeld, L. Pérez, J.F. Asturiano

Fish Physiology and Biochemistry, 44(6): 1457-1467
Doi: 10.1007/s10695-018-0505-1

Testing cryopreserved European eel sperm for hybridization (A. japonica × A. anguilla)

T. Müller, H. Matsubara, Y. Kubara, Á. Horváth, B. Kolics, J. Taller, V. Stéger, B. Kovács, L. Horváth, J.F. Asturiano, D.S. Peñaranda, D. Kucharczyk, B. Urbányi

Theriogenology, 113: 153-158

Doi: 10.1016/j.theriogenology.2018.02.021

Handling and treatment of male European eels (Anguilla anguilla) for hormonal maturation and sperm cryopreservation 

J.G. Herranz-Jusdado, E. Kása, T. Kollár, V. Gallego, D.S. Peñaranda, C. Rozenfeld, L. Pérez, Á. Horváth, J.F. Asturiano

Journal of Visualized Experiments, 131:e56835

Doi: 10.3791/56835

Sperm motility in fish: technical applications and perspectives through CASA-Mot systems

V. Gallego, J.F. Asturiano

Reproduction, Fertility and Development, 30(6): 820-832

Doi: 10.1071/RD17460

Using specific recombinant gonadotropins to induce spermatogenesis and spermiation in the European eel (Anguilla anguilla)

D.S. Peñaranda, V. Gallego, C. Rozenfeld, J.G. Herranz-Jusdado, L. Pérez, A. Gómez, I. Giménez, J.F. Asturiano

Theriogenology, 107: 6-20

Doi: 10.1016/j.theriogenology.2017.11.002

Available here: https://authors.elsevier.com/a/1W0~P,28LgwAeO

2017

The expression of nuclear and membrane estrogen receptors in the European eel throughout spermatogenesis

M. Morini, D.S. Peñaranda, M.C. Vílchez, H. Tveiten, A.G. Lafont, S. Dufour, L. Pérez, J.F. Asturiano

Comparative Biochemistry and Physiology - Part A: Molecular & Integrative Physiology, 203: 91-99. 2017

Doi:10.1016/j.cbpa.2016.08.020
Available here: http://authors.elsevier.com/a/1Thk-3v7jMgp13

Role of potassium and pH on the initiation of sperm motility in the European eel

M.C. Vílchez, M. Morini, D.S. Peñaranda, V. Gallego, J.F. Asturiano, L. Pérez

Comparative Biochemistry and Physiology - Part A: Molecular & Integrative Physiology

Doi: 10.1016/j.cbpa.2016.09.024
Available here:  http://authors.elsevier.com/a/1TsEw3v7jMgp6i 

Embryonic development of the grass pufferfish (Takifugu niphobles): From egg to larvae

V. Gallego, M. Yoshida, D. Kurokawa, J.F. Asturiano, G.J. Fraser

Theriogenology, 90: 191-196

Doi: 10.1016/j.theriogenology.2016.12.005
Available here: http://www.theriojournal.com/article/S0093-691X(16)30579-9/pdf

Japanese eel (Anguilla japonica Temminck & Schlegel, 1846) propagation by using cryopreserved sperm samples

T. Müller, H. Matsubara­, Y. Kubara, Á. Horváth, J.F. Asturiano, B. Urbányi

Journal of Applied Ichthyology, 33: 550-552
Doi: 10.1111/jai.13316 
Available here: http://onlinelibrary.wiley.com/doi/10.1111/jai.13316/epdf

Nuclear and membrane progestin receptors in the European eel: characterization and expression in vivo through spermatogenesis

M. Morini, D.S. Peñaranda, M.C. Vílchez, R. Nourizadeh-Lillabadi, A.G. Lafont., S. Dufour, J.F. Asturiano, F.-A. Weltzien, L. Pérez

Comparative Biochemistry and Physiology - Part A: Molecular & Integrative Physiology, 207: 79-92
doi: 10.1016/j.cbpa.2017.02.009

Available here:http://dx.doi.org/10.1016/j.cbpa.2017.02.009 

5th International Workshop on the Biology of Fish Gametes – Evidences of a series growing in numbers and quality

J.F. Asturiano, Á. Horváth, O. Carnevali
General and Comparative Endocrinology, 245: 1-4
doi: 10.1016/j.ygcen.2017.03.004
Available here:  http://www.sciencedirect.com/science/article/pii/S0016648017301788 

Progress, challenges and perspectives on fish gamete cryopreservation: A mini-review

J.F. Asturiano, E. Cabrita, Á. Horváth

General and Comparative Endocrinology, 245: 69-76
doi:10.1016/j.ygcen.2016.06.01:9
Available here: http://dx.doi.org/10.1016/j.ygcen.2016.06.019

Development of sperm vitrification protocols for freshwater fish (Eurasian perch, Perca fluviatilis) and marine fish (European eel, Anguilla anguilla)

E. Kása, G. Bernáth, T. Kollár, D. Żarski, J. Lujić, Z. Marinović, Z. Bokor, Á. Hegyi, B. Urbányi, M.C. Vílchez, M. Morini, D.S. Peñaranda, L. Pérez, J.F. Asturiano, Á. Horváth

General and Comparative Endocrinology, 245: 102-107
doi:10.1016/j.ygcen.2016.05.010

Available here: http://www.sciencedirect.com/science/article/pii/S0016648016301319
 

Hormonal manipulations for the enhancement of sperm production in cultured fish and evaluation of sperm quality

C.C. Mylonas, N. Duncan, J.F. Asturiano

Aquaculture, 472: 21-44
Doi: 10.1016/j.aquaculture.2016.04.021
Available here: http://dx.doi.org/10.1016/j.aquaculture.2016.04.021 

Molecular markers of oocyte differentiation in European eel during hormonally induced oogenesis
I. Rojo-Bartolomé, L. Martínez-Miguel, A.G. Lafont, M.C. Vílchez, J.F. Asturiano, L. Pérez, I. Cancio
Comparative Biochemistry and Physiology - Part A: Molecular & Integrative Physiology, 2011: 17-25
Doi: 10.1016/j.cbpa.2017.05.018
Available here: http://www.sciencedirect.com/science/article/pii/S1095643317301216

2016

Temperature modulates testis steroidogenesis in European eel

D.S. Peñaranda, M. Morini, H. Tveiten, M.C. Vílchez, V. Gallego, R.P. Dirks, G.E.E.J.M. van den Thillart, L. Pérez, J.F. Asturiano
Comparative Biochemistry and Physiology - Part A: Molecular & Integrative Physiology, 197: 58-67. 2016
Doi: 10.1016/j.cbpa.2016.03.012
Available here: http://authors.elsevier.com/a/1SotW3v7jMgoit

Role of calcium on the initiation of sperm motility in the European eel

L. Pérez, M.C. Vílchez, V. Gallego, M. Morini, D.S. Peñaranda, J.F. Asturiano

Comparative Biochemistry and Physiology Part A: Molecular & Integrative Physiology , 191: 98-106. 2016

Doi: 10.1016/j.cbpa.2015.10.009

Sodium affects the sperm motility in the European eel

M.C. Vílchez, M. Morini, D.S. Peñaranda, V. Gallego, J.F. Asturiano, L. Pérez

Comparative Biochemistry and Physiology Part A: Molecular & Integrative Physiology, 198: 51-58. 2016
Doi: 10.1016/j.cbpa.2016.04.008
Available here: http://authors.elsevier.com/a/1SwLF3v7jMgon9

First production of larvae using cryopreserved sperm: Effects of preservation temperature and cryopreservation on European eel sperm fertilization capacity

J.F. Asturiano, S.R. Sørensen, L. Pérez, P. Lauesen, J. Tomkiewicz
Reproduction in Domestic Animals, 51: 485-491. 2016
Doi: 10.1111/rda.12706
Available on line: http://onlinelibrary.wiley.com/doi/10.1111/rda.12706/abstract

Identification of the major proteins present in the seminal plasma of European eel, and how hormonal treatment affects their evolution. Correlation with sperm quality

M.C. Vílchez, D. Pla, V. Gallego, L. Sanz, L. Pérez, J.F. Asturiano, J.J. Calvete, D.S. Peñaranda

Comparative Biochemistry and Physiology - Part A: Molecular & Integrative Physiology, 201: 37-45. 2016

Doi:10.1016/j.cbpa.2016.06.025

Available here: http://dx.doi.org/10.1016/j.cbpa.2016.06.025

A comparison of techniques for studying oogenesis in the European eel Anguilla anguilla

I. Mazzeo, E. Giorgini, G. Gioacchini, F. Maradonna, M.C. Vílchez, S. Baloche, S. Dufour, L. Pérez, O. Carnevali, J.F. Asturiano
Journal of Fish Biology, 89: 2055-2069. 2016
Doi:10.1111/jfb.13103

2015

Effect of the probiotic Lactobacillus rhamnosus on the expression of genes involved in European eel spermatogenesis

M.C. Vílchez, S. Santangeli, F. Maradonna, G. Gioacchini, C. Verdenelli, V. Gallego, D.S. Peñaranda, H. Tveiten, L. Pérez, O. Carnevali, J.F. Asturiano
Theriogenology, 84: 1321-1331. 2015
Doi: 10.1016/j.theriogenology.2015.07.011

Transcript levels of the soluble sperm factor protein phospholipase C zeta 1 (PLCζ1) increase through induced spermatogenesis in European eel

M. Morini, D.S. Peñaranda, M.C. Vílchez, V. Gallego, R. Nourizadeh-Lillabadi, J.F. Asturiano, F.-A. Weltzien, L. Pérez

Comparative Biochemistry and Physiology - Part A: Molecular & Integrative Physiology 187: 168-176. 2015
Doi: 10.1016/j.cpba.2015.05.028

Foreword. The Fourth International Workshop on the Biology of Fish Gametes, 17-20 September 2013, Albufeira, Algarve, Portugal: Summary and conclusions

H. Rosenthal, J.F. Asturiano, Á. Horváth, S. Martínez-Páramo

Journal of Applied Ichthyology 31 (Suppl. 1): 1-2. 2015
Doi: 10.1111/jai.1271

Exploring correlations between sex steroids and fatty acids and their potential roles in induced maturation of male European eel

R. Baeza, D.S. Peñaranda, M.C. Vílchez, H. Tveiten, L. Pérez, J.F. Asturiano

Aquaculture 435: 328-335. 2015

doi: 10.1016/j.aquaculture.2014.10.016

Relationship between sperm quality parameters and the fatty acid composition of the muscle, liver and testis of European eel

R. Baeza, I. Mazzeo, M.C. Vílchez, V. Gallego, D.S. Peñaranda, L. Pérez, J.F. Asturiano

Comparative Biochemistry and Physiology - Part A: Molecular & Integrative Physiology  181: 79-86. 2015

doi: 10.1016/j.cbpa.2014.11.022

Impact of dietary fatty acids on muscle composition, liver lipids, milt composition and sperm performance in European eel

I.A.E. Butts, R. Baeza, J.G. Støttrup, M. Krüger-Johnsen, C. Jacobsen, L. Pérez, J.F. Asturiano, J. Tomkiewicz

Comparative Biochemistry and Physiology - Part A: Molecular & Integrative Physiology 183: 87-96. 2015
doi: 10.1016/j.cbpa.2015.01.015

The subpopulation pattern of eel sperm is affected by post-activation time, hormonal treatment and thermal regime

V. Gallego, M.C. Vílchez, D.S. Peñaranda, L. Pérez, M.P. Herráez, J.F. Asturiano, F. Martínez-Pastor

Reproduction, Fertility and Development 27(3) 529-543. 2015

doi: 10.1071/RD13198

2014

Intracellular changes in Ca2+, K+ and pH after sperm motility activation in the European eel (Anguilla anguilla): Preliminary results
V. Gallego; F. Martínez-Pastor;  I. Mazzeo; D.S. Peñaranda; M.P. Herráez; J.F. Asturiano; L. Pérez

Aquaculture 418-419: 155-158. 2014

http://dx.doi.org/10.1016/j.aquaculture.2013.10.022

Larval width as indicator of growth rate and effect of larval classification on final body composition and flesh quality in cultured gilthead seabream (Sparus aurata, L.)

I. Mazzeo, Y.J. Borrell, V. Gallego, C. García Fernández, J.A. Sánchez, G. Blanco, L. Pérez, J.F. Asturiano

Journal of Applied Ichthyology 30: 300-306. 2014
doi: 10.1111/jai.12380

The regulation of aromatase and androgen receptor expression during gonad development in male and female European eel

D.S. Peñaranda, I. Mazzeo, V. Gallego, J. Hildahl, R. Nourizadeh-Lillabadi, L. Pérez, F.-A. Weltzien, J.F. Asturiano
Reproduction in Domestic Animals. 49(3): 512-521. 2014
doi: 10.1111/rda.12321

Effect of thermal regime on fatty acid dynamics in male European eels (Anguilla anguilla) during hormonally-induced spermatogenesis
R. Baeza, I. Mazzeo, M.C. Vílchez, V. Gallego, D.S. Peñaranda, L. Pérez, J.F. Asturiano
Aquaculture. 430: 86-97. 2014
doi: 10.1016/j.aquaculture.2014.03.045

Sperm motility parameters and spermatozoa morphometric characterization in marine species: a study of swimmer and sessile species
V. Gallego, L. Pérez, J.F. Asturiano, M. Yoshida
Theriogenology. 82(5): 668-676. 2014
doi: 10.1016/j.theriogenology.2014.05.026

http://authors.elsevier.com/a/1PhAM,28LgjaKQ

Temperature modulates the vitellogenesis progression in European eel
I. Mazzeo; D.S. Peñaranda; V. Gallego; S. Baloche, R. Nourizadeh-Lillabadi; H. Tveiten, S. Dufour, J.F. Asturiano; F.A. Weltzien; L. Pérez
Aquaculture. 434: 38-47. 2014
doi: 10.1016/j.aquaculture.2014.07.020



2013

Molecular characterization of three GnRH receptor paralogs in the European eel, Anguilla anguilla: Tissue-distribution and changes in transcript abundance during artificially induced sexual development
D.S. Peñaranda; I. Mazzeo; J. Hildahl; V. Gallego; R. Nourizadeh-Lillabadi; L. Pérez; J.F. Asturiano; F.A. Weltzien
Molecular and Cellular Endocrinology 369(1-2): 1-14. 2013
doi:10.1016/j.mce.2013.01.025

Standardization of European eel (Anguilla anguilla) sperm motility evaluation by CASA software
V. Gallego; P.C.F. Carneiro; I. Mazzeo; M.C. Vílchez; D.S. Peñaranda; C. Soler; L. Pérez; J.F. Asturiano

Theriogenology 79: 1034-1040. 2013
doi:10.1016/j.theriogenology.2013.01.019

Study of puffer fish (Takifugu niphobles) sperm: development of methods for short-term storage, effects of different activation media and role of intracellular changes in Ca2+ and K+ in the initiation of sperm motility.

V. Gallego; L. Pérez; J.F. Asturiano; M. Yoshida

Aquaculture 414-415: 82-91

doi: 10.1016/j.aquaculture.2013.07.046

Relationship between spermatozoa motility parameters, sperm/egg ratio, and fertilization and hatching rates in pufferfish (Takifugu niphobles)
V. Gallego, L. Pérez, J.F. Asturiano; M. Yoshida
Aquaculture 416-417: 238-243. 2013
doi:10.1016/j.aquaculture.2013.08.035

Evaluation of methods to determine sperm density for the European eel, Anguilla anguilla

S.R. Sørensen; V. Gallego; L. Pérez; I.A.E. Butts; J. Tomkiewicz; J.F. Asturiano
Reproduction in Domestic Animals 48(6): 936-944. 2013
doi:10.1111/rda.12189

El futuro de la producción de la anguila: mejoras en la reproducción  

M. Jover; J.F. Asturiano; L. Pérez

Foro Rec. Mar. Ac. Rías Gal. 15: 41-54 (En prensa)

  

2012

Comparison of two techniques for the morphometry study on gilthead seabream (Sparus aurata) spermatozoa and evaluation of changes induced by cryopreservation

V. Gallego; D.S. Peñaranda; F. Marco-Jiménez; I. Mazzeo; L. Pérez; J.F. Asturiano

Theriogenology 77: 1078-1087. 2012

doi:10.1016/j.theriogenology.2011.10.010

Study of the effects of thermal regime and alternative hormonal treatments on the reproductive performance of European eel males (Anguilla anguilla) during induced sexual maturation

V. Gallego; I. Mazzeo; M.C. Vílchez; D.S. Peñaranda; P.C.F. Carneiro; L. Pérez; J.F. Asturiano

Aquaculture 354-355: 7-16. 2012

doi:10.1016/j.aquaculture.2012.04.041

Variations in the gene expression of zona pellucida proteins, zpb and zpc, in female European eel (Anguilla anguilla) during induced sexual maturation

I. Mazzeo; D.S. Peñaranda; V. Gallego; J. Hildahl; R. Nourizadeh-Lillabadi; J.F. Asturiano; L. Pérez; F.A. Weltzien

General and Comparative Endocrinology 178(2): 338-346. 2012

doi:10.1016/j.ygcen.2012.06.003

Trying to reproduce the European eel (Anguilla anguilla) under captivity: experiments with females

L. Pérez; M.C. Vílchez; V. Gallego; I. Mazzeo; D.S. Peñaranda; F.-A. Weltzien; S. Dufour; J.F. Asturiano

En: Domestication in Finfish Aquaculture. Páginas: 11-15. 2012

Monográfico Innovafish. Pracownia Wydawnicza “ElSet”, Olsztyn (Poland)

On the biology of fish gametes: summary and recommendations of the Third International Workshop, Budapest and Gödöllo, Hungary, 2011

Á. Horváth; J.F. Asturiano; H. Rosenthal

Journal of Applied Ichthyology 28(6): 863-864. 2012

doi:10.1111/jai.12099

Temperature effect on expression of P450 aromatase, zona pellucida proteins and androgen receptors, and plasma levels of E2 in female European eel during induced sexual maturation

I. Mazzeo; D.S. Peñaranda; V. Gallego; R. Nourizadeh-Lillabadi; H. Tveiten; F.A. Weltzien; J.F. Asturiano; L. Pérez

En: Avances en Endocrinología Comparada, Vol. VI. Páginas: 125-128. 2012

Asociación Ibérica de Endocrinología Comparada (Editado por M.J. Delgado, Á.L. Alonso, N. de Pedro y E. Isorna)

Regulation of gene expression of CYP19A, GnRH receptors I and II, and androgen receptors a and b in male European eel (Anguilla anguilla) during induced sexual maturation

D.S. Peñaranda; I. Mazzeo; V. Gallego; R. Nourizadeh-Lillabadi; F.A. Weltzien; L. Pérez; J.F. Asturiano

En: Avances en Endocrinología Comparada, Vol. VI. Páginas: 222-224. 2012

Asociación Ibérica de Endocrinología Comparada (Editado por M.J. Delgado, Á.L. Alonso, N. de Pedro y E. Isorna)

2011

 

Assessment of parental contributions to fast and slow growth progenies in the sea bream Sparus aurata L. using a new multiplex PCR

Y. Borrell; V. Gallego; C. García Fernández; I. Mazzeo; L. Pérez; J.F. Asturiano; C.E. Carleos; E. Vázquez; J.A. Sánchez; G. Blanco
Aquaculture 314: 58-65. 2011
doi:10.1016/j.aquaculture.2011.01.028
 
Influence of temperature regime on endocrine parameters and vitellogenesis during experimental maturation of European eel (Anguilla anguilla) females
L. Pérez; D.S. Peñaranda; S. Dufour; S. Baloche; A.P. Palstra; G.E.E.J.M. van den Thillart; J.F. Asturiano
General and Comparative Endocrinology 174: 51-59. 2011
doi: 10.1016/ygcen.2011.08.009

Heterozygosity-fitness correlations in the gilthead Sparus aurata using microsatellite loci from unknown and gene-rich genomic locations

Y.J. Borrell; J.A. Sánchez; E. Vázquez; C.E. Carleos; V. Gallego; J.F. Asturiano; G. Blanco
Journal of Fish Biology 79: 1111-1129. 2011
doi:10.1111/j.1095-8649.2011.03099.x
 

2010

Molecular and physiological study of the artificial maturation process in the European eel males: from brain to testis
D.S. Peñaranda; L. Pérez; V. Gallego; M. Jover; S. Baloche; S. Dufour; J.F. Asturiano
General and Comparative Endocrinology 166: 160-171. 2010

European eel sperm diluent for short-term storage

D.S. Peñaranda; L. Pérez; V. Gallego; R. Barrera; M. Jover; J.F. Asturiano
Reproduction in Domestic Animals 45: 407-415. 2010
 

On the biology of fish gametes: summary and recommendations of the Second International Workshop, Valencia, Spain, 2009

H. Rosenthal; J.F. Asturiano; O. Linhart; A. Horvath
Journal of Applied Ichthyology 26: 621-622. 2010
 

Variations on fatty acids composition in different tissues of European eel (Anguilla anguilla) males during induced sexual maturation

I. Mazzeo; V. Gallego; L. Pérez; D.S. Peñaranda; M. Jover; J.F. Asturiano
Journal of Applied Ichthyology 26: 763-774. 2010
 

Protein profile study in European eel (Anguilla anguilla) seminal plasma and its correlation with sperm quality

D.S. Peñaranda; F. Marco-Jiménez; V. Gallego; I. Mazzeo; L. Pérez; M. Jover; J.F. Asturiano
Journal of Applied Ichthyology 26: 746 -752. 2010
 

Evaluation of different diluents for short-term storage of European eel sperm under air-limited conditions

D.S. Peñaranda; F. Marco-Jiménez; L. Pérez; V. Gallego; I. Mazzeo; J.S. Vicente; M. Jover; J.F. Asturiano
Journal of Applied Ichthyology 26: 659-664. 2010

2009

Different protocols for the cryopreservation of European eel (Anguilla anguilla) sperm
J.F. Asturiano

En: Methods in Reproductive Aquaculture: Marine and Freshwater Species
(Editado por Cabrita E., Robles V., Herráez M.P.)
CRC Press. Taylor & Francis Group. Páginas: 415-420. 2009

Testis development, sperm quality evaluation and cryopreservation in the European eel

L. Pérez; D.S. Peñaranda; V. Gallego; J.F. Asturiano
En: Spawning migration of the European Eel
(Editado por G. van den Thillart, S. Dufour y C. Rankin)
Springer. Fish and Fisheries Series 30. Chapter 14: 333-362. 2009

Improvement of European eel sperm cryopreservation method by preventing spermatozoa movement activation caused by cryoprotectants

D.S. Peñaranda; L. Pérez; V. Gallego; M. Jover; J.F. Asturiano
Cryobiology 59: 119-126. 2009

Book of abstracts. Second International Workshop on Biology of Fish Gametes

Varios (J.F. Asturiano: editor)
Páginas: 145. 2009

2008

Effects of pH, sodium bicarbonate, cryoprotectants and foetal bovine serum on the cryopreservation of European eel sperm
D.L. Garzón; D.S. Peñaranda; L. Pérez; F. Marco-Jiménez; X. Espert; T. Müller; M. Jover; J.F. Asturiano
Reproduction in Domestic Animals 43: 99-105. 2008
 

Effects of extenders and cryoprotectant combinations on motility and morphometry of sea bass (Dicentrarchus labrax) spermatozoa

D.S. Peñaranda; L. Pérez; G. Fakriadis; C.C. Mylonas; J.F. Asturiano
Journal of Applied Ichthyology 24: 450-455. 2008
 

Morphometric characterisation of sharpsnout sea bream (Diplodus puntazzo) and gilthead sea bream (Sparus aurata) spermatozoa using computer-assisted spermatozoa analysis (ASMA)

F. Marco-Jiménez; D.S. Peñaranda; L. Pérez; M.P. Viudes-de-Castro; C.C. Mylonas; M. Jover; J.F. Asturiano
Journal of Applied Ichthyology 24: 382-385. 2008
 

Results of maturation and ovulation in European eel females

L. Pérez; D.S. Peñaranda; M. Jover; J.F. Asturiano
Cybium, International Journal of Ichthyology 32(2) suppl.: 320. 2008
 
Advances on the techniques for the control of the European eel reproduction: spermiation induction, sperm quality evaluation and cryopreservation
D.S. Peñaranda, L. Pérez, F. Marco-Jiménez, M. Jover, J.F. Asturiano
Cybium, International Journal of Ichthyology 32(2) suppl.: 323. 2008
 

Variaciones de las GnRHs cerebrales y de la expresión de gonadotropinas en la hipófisis de machos de anguila europea en relación con la maduración artificial de la gónada

D.S. Peñaranda; L. Pérez; X. Espert; V. Gallego; S. Baloche; M. Jover; S. Dufour; J.F. Asturiano
En: Avances en Endocrinología Comparada, Vol. IV.
(Editado por Muñoz-Cueto J.A., Mancera J.M., Martínez-Rodríguez G.)
Páginas: 339-342. 2008
 

Expresión de FSHb y LHb en hembras de anguila europea maduradas hormonalmente bajo dos regímenes de temperatura

D.S. Peñaranda; J.F. Asturiano; S. Baloche; M. Jover; S. Dufour; L. Pérez
En: Avances en Endocrinología Comparada, Vol. IV.
(Editado por Muñoz-Cueto J.A., Mancera J.M., Martínez-Rodríguez G.)
Páginas: 343-346. 2008