Characterization and environmental stress-induced
expression profiling of transient receptor potential vanilloid (TRPV) channels
in the Pacific oyster (Magallana gigas) following short-heatwave and
silver exposure
Fátima Fernández-García, Cláudia Mieiro, Mario Pacheco, Juan F. Asturiano, Marina Morini
Abstract
Marine
ecosystems are increasingly threatened by the rising frequency of thermal
anomalies, such as marine heatwaves (HW), and by the presence of emerging
contaminants, including silver nanoparticles (Ag NPs) and its dissociated form
into silver ions (Ag⁺).
In this context, the present study provides an integrative analysis of the
molecular basis of Transient Receptor Potential Vanilloid (TRPV), a family of
sensitive channels, under environmentally realistic conditions in the gonad of
the Pacific oyster (Magallana gigas). In this study, environmentally relevant
concentrations of silver (0.25 μg/L) and a temperature increase of up to 6 °C
above natural temperature were applied to simulate a realistic short-HW
scenario. Four TRPV sequences were identified and, based on phylogenetic
analyses, reclassified as TRPVA, TRPVB, and two TRPVC/D paralogs. Among these,
TRPVB emerged as the most stress-responsive isoform in the gonad, displaying
contrasting expression profiles under HW conditions, with Ag NPs upregulating
TRPVB expression whereas Ag⁺
caused its downregulation. Although preliminary, these results suggest that Ag
NPs and Ag⁺ activate
different signalling pathways, which may be differentially expressed under
thermal stress, highlighting the complexity of combined contaminants and
climate-related pressures in the Pacific oyster. These findings provide basic
knowledge and novel insights into environmental stress responses in marine
invertebrates.
Comparative Biochemistry and Physiology, Part C: Toxicology and Pharmacology, 304: 110477
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