Temperature modulates testis steroidogenesis in European eel
David
S. Peñaranda, Marina Morini, Helge Tveiten, M. Carmen Vílchez, Víctor Gallego,
Ron P. Dirks, Guido E.E.J.M. van den Thillart, Luz Pérez, Juan F. Asturiano
Abstract
This
study evaluates the effects of temperature on hCG-induced spermatogenesis in
European eel (Anguilla anguilla),
subjected to three thermal regimes: T10: 10 °C (first 4 weeks), 15 °C (next 3
weeks) and 20 °C (last 6 weeks); T15: 15 °C (first 4 weeks) and 20 °C (last 9
weeks); and T20: constant 20 °C for the duration of the experiment. At 10 ºC,
maturation stopped in the A spermatogonial stage (SPG1), and no further
maturation was observed until the temperature was ≥15 ºC. With the aim of
explaining these results, the influence of temperature on steroidogenic enzyme
gene expression and steroid synthesis was tested. The initial synthesis of
androgens (T and 11-KT) increased at SPG1, and was not influenced by
temperature. Likewise, the gene expression of the steroidogenic enzymes linked
to androgen synthesis (aacyp11a1,
aacyp17-I and aa11βHSD) also increased at SPG1. In contrast, no correlation
was seen between the increase in E2 and the aacyp19a1
gene expression peak in the testes, with E2 increasing as a consequence of the
seawater acclimation carried out before hormonal treatment, and peaking the aacyp19a1 gene expression at B
spermatogonial stage (SPG2). Aacyp21 gene expression was also higher
at SPG2, and this stage was only reached when the rearing temperature was ≥15
ºC.
In
conclusion, androgen synthesis is not dependent on temperature, but further
maturation requires higher temperatures in order to induce a change in the
steroidogenic pathway towards oestrogen and progestin synthesis. This study
demonstrates that temperature plays a crucial role in European eel maturation,
even perhaps controlling gonad development during the reproductive migration.
Available here: http://authors.elsevier.com/a/1SotW3v7jMgoit
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