Nuestro último artículo, publicado en Aquaculture

 

Species-specific responses of fish sperm to thermal incubation and activation during short-term storage

Songpei Zhang, Shuang Peng, Yu Gao, Zhijun Ma, Thales S. França, Yu Cheng, Nururshopa Eskander Shazada, Fátima Fernández-García, Jaime Pérez-Sánchez, Juan F. Asturiano, Serhii Boryshpolets, Marek Rodina, Otomar Linhart, Zuzana Havlíková

Abstract

Sperm samples from six species: zebrafish (Danio rerio), common carp (Cyprinus carpio L.), European eel (Anguilla anguilla), gilthead seabream (Sparus aurata), sterlet (Acipenser ruthenus) and rainbow trout (Oncorhynchus mykiss), were accordingly diluted with immobilising solutions and stored at 0-2 °C under aerobic conditions. The effect of the following incubation and activation at low (4 °C) and relatively high temperatures (natural species-specific spawning temperature) on various spermatozoa motility parameters during the storage was estimated. During sperm storage, sperm motility and velocity declined when activated at the species-specific spawning temperature. Nevertheless, incubation and activation at higher temperatures were associated with improved motility parameters in several species. Significant variations were observed between different species in sperm motility and velocity during storage, particularly in response to low- or high-temperature incubation and activation. These observations were reflected in distinct clustering patterns of sperm motility parameters of stored sperm in zebrafish, common carp, and sterlet. In contrast, European eel and gilthead seabream did not exhibit clear clustering, reflecting interspecies variations. GLMM analysis revealed that sperm motility parameters were generally affected, albeit to varying degrees, by thermal conditions during incubation and activation. Further validation using an XGBoost model revealed that storage duration, incubation, and activation temperatures markedly impacted sperm motility in thermophilic and mesophilic fish species (zebrafish, common carp, and European eel) compared to the other species. These results underscore the broad applicability and effectiveness of chilled sperm storage at 0-2 °C for preserving sperm quality across diverse fish species under variable thermal incubation and activation conditions.

doi: 10.1016/j.aquaculture.2025.742971