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y en especial con las líneas de investigación en reproducción de peces del Grupo de Acuicultura y Biodiversidad
de la Universitat Politècnica de València.
(Foto: Joseph Wu Origami)
Nuestro último artículo, aceptado en Theriogenology
Intracellular pH regulation and sperm motility
in the European eel
L. Pérez, V.
Gallego, J.F. Asturiano
activation involves ion fluxes as well as a previous capacitation in the seminal
plasma, something which has not been studied in depth in marine fish species.
pH and potassium are probably involved in sperm capacitation and motility in
the European eel, as indicated in previous studies. In this work, the absolute
intracellular concentration of potassium in European eel sperm has been
determined for the first time. In addition, the intracellular pH (pHi) of
quiescent eel spermatozoa was determined by two methods (nigericin and null
point) that gave similar results, 7.4-7.6. The natural pHi range of sperm
samples in the quiescent stage was 7.4-8.0, with no evident relationship with
sperm motility (percentage of motile cells) once the sperm samples were activated.
However, a linear correlation was seen between sperm motility and the pH of the
diluent or extracellular pH (pHe), as well as between the pHi and the pH of the
diluent. The pHi change post-activation in seawater (ASW) depended on the
initial pHe of the diluent medium.
with ASW induced an internal alkalinization of the cells when the sample had
previously been diluted in a pHe<8.0; an acidification when pHe>8, and no
pHi variation when pHe was 8.0. These experiments indicated that a careful
selection of the diluents should be performed before measuring natural pHi
changes in sperm cells. Thus, studies on the specific seminal plasma
composition of marine fish species are necessary before studying their
physiology. Furthermore, our study indicates that intracellular alkalinization
is not a universal fact during sperm activation. Theriogenology, 145: 48-58 doi: 10.1016/j.theriogenology.2020.01.026